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 Indian J Med Microbiol  
 

Figure 6: Western immunoblotting using antibody recognizing general phosphorylated tyrosine as an indicator of sperm capacitation after hydrogen peroxide treatment. The result shows reduced band intensity along with increasing concentration of hydrogen peroxide from 50 to 250 μM. The major bands detected in this analysis were AKAP4 (82 kDa) and AKAP3 (110 kDa). Membrane was stripped and re-probed with tubulin antibody as a loading control. The experiment was repeated at least three times with the same result. M = Protein marker, kDa

Figure 6: Western immunoblotting using antibody recognizing general phosphorylated tyrosine as an indicator of sperm capacitation after hydrogen peroxide treatment. The result shows reduced band intensity along with increasing concentration of hydrogen peroxide from 50 to 250 μM. The major bands detected in this analysis were AKAP4 (82 kDa) and AKAP3 (110 kDa). Membrane was stripped and re-probed with tubulin antibody as a loading control. The experiment was repeated at least three times with the same result. M = Protein marker, kDa