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 Indian J Med Microbiol  
 

Figure 2: Polymerase chain reaction amplicons of size 162 base pairs (bp) generated from COL4A1 gene and harboring the c.1537G>A mutation. Lane 1: 100 bp DNA marker; Lane 2-8: Amplicons generated from whole genome amplified DNA originating from cells of seven different representative embryos screened in this study; Lane 9: Amplicons generated from blood DNA of father who is heterozygous for the pathogenic COL4A1 gene allele; Lane 10: No template control

Figure 2: Polymerase chain reaction amplicons of size 162 base pairs (bp) generated from COL4A1 gene and harboring the c.1537G>A mutation. Lane 1: 100 bp DNA marker; Lane 2-8: Amplicons generated from whole genome amplified DNA originating from cells of seven different representative embryos screened in this study; Lane 9: Amplicons generated from blood DNA of father who is heterozygous for the pathogenic COL4A1 gene allele; Lane 10: No template control