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 Indian J Med Microbiol  
 

Figure 5: Preparation of Microdroplets. A 50 × 09 mm Petri dish containing several microdroplets of culture medium under mineral oil is prepared for sperm pick-up from a processed epididymal or testicular cell suspension. Microdroplets are prepared as follows: Four 10-μL sperm medium at dish periphery to load specimens (numbered 1 to 4), one 4-μL polyvinylpyrrolidone (PVP) at dish center in a triangle shape to pick up selected sperm for ICSI (number 5), and two to three 10-μL sperm medium at dish center below the PVP triangle for washing (numbered 6 to 8). Alternatively, one of the sperm medium-containing microdroplets (e.g., number 8) or the peripheric ones (1-4) may be replaced with the hyposmotic or motility-stimulant solutions, respectively (left). The hyposmotic swelling test is illustrated (right). The sperm tail is partially withdrawn from the injection micropipette into the HOS droplet. A swelling at the level of the tail tip may be seen under the inverted microscope with contrast at ×400 magnification

Figure 5: Preparation of Microdroplets. A 50 × 09 mm Petri dish containing several microdroplets of culture medium under mineral oil is prepared for sperm pick-up from a processed epididymal or testicular cell suspension. Microdroplets are prepared as follows: Four 10-μL sperm medium at dish periphery to load specimens (numbered 1 to 4), one 4-μL polyvinylpyrrolidone (PVP) at dish center in a triangle shape to pick up selected sperm for ICSI (number 5), and two to three 10-μL sperm medium at dish center below the PVP triangle for washing (numbered 6 to 8). Alternatively, one of the sperm medium-containing microdroplets (e.g., number 8) or the peripheric ones (1-4) may be replaced with the hyposmotic or motility-stimulant solutions, respectively (left). The hyposmotic swelling test is illustrated (right). The sperm tail is partially withdrawn from the injection micropipette into the HOS droplet. A swelling at the level of the tail tip may be seen under the inverted microscope with contrast at ×400 magnification