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 Indian J Med Microbiol  
 

Figure 3: Laboratory processing of testicular specimens. (a) A fragment of testicular parenchyma obtained by microdissection testicular sperm extraction. (b) Testicular parenchyma fragment examined under stereomicroscopy before processing using ×40 magnification. (c) Seminiferous tubules examined under steromicroscopy using ×40 magnification. Observe the difference in diameter and appearance of the loosen tubules (arrows). (d) Mechanical mincing of the seminiferous tubules using needled-tuberculin syringes. (e) Aspect of the cell suspension after mechanical mincing of the tubules. No intact seminiferous tubule is seen. (f) Processed specimen examined under the inverted microscope using ×400 magnification. Testicular sperm were identified as shown

Figure 3: Laboratory processing of testicular specimens. (a) A fragment of testicular parenchyma obtained by microdissection testicular sperm extraction. (b) Testicular parenchyma fragment examined under stereomicroscopy before processing using ×40 magnification. (c) Seminiferous tubules examined under steromicroscopy using ×40 magnification. Observe the difference in diameter and appearance of the loosen tubules (arrows). (d) Mechanical mincing of the seminiferous tubules using needled-tuberculin syringes. (e) Aspect of the cell suspension after mechanical mincing of the tubules. No intact seminiferous tubule is seen. (f) Processed specimen examined under the inverted microscope using ×400 magnification. Testicular sperm were identified as shown