Journal of Human Reproductive Sciences

ORIGINAL ARTICLE
Year
: 2021  |  Volume : 14  |  Issue : 2  |  Page : 191--195

Cytogenetic screening in couples with recurrent pregnancy loss: A single-center study and review of literature


Rim Frikha, Fatma Turki, Nouha Abdelmoula, Tarek Rebai 
 Department of Medical Genetics, CHU Hedi Chaker; Laboratory of Histology, Faculty of Medicine, University of Sfax-, Tunisia

Correspondence Address:
Dr. Rim Frikha
Department of Medical Genetics and Laboratory of Histology, Faculty of Medicine, University of Sfax
Tunisia

Context: Recurrent pregnancy loss (RPL) is a devastating reproductive problem that affects more than 2% of couples who are trying to conceive. Chromosomal rearrangements in either carrier are a major cause of clinically recognized abortion. Aims: The purpose of this study is to report the prevalence of chromosome abnormalities in RPL and provide clinical characteristics of couples with two and more miscarriages. Settings and Design: Genetic counseling in laboratory of histology housed in a Faculty of Medicine of Sfax. Materials and Methods: Karyotype was generated from the peripheral blood lymphocyte cultures and the cytogenetic analysis was performed using R-bands after heat denaturation and Giemsa (RHG) banding. A multiplex polymerase chain reaction wherever necessary was done. Statistical Analysis Used: SPSS version 17. Results: A total of 104 couples with RPL were carried out in this study. The frequency of chromosomal rearrangements was 11.5%, three times more prevalent in men than women (P = 0.08). In addition, the prevalence of chromosomal anomalies increases according to the number of miscarriages (from 4.8% to 7.6%, with 2 or ≥3 miscarriages, respectively; P = 0.9). Finally, a particular familial adverse reproductive background was found in these carriers (P = 0.03). Conclusions: These data highlight that an RPL evaluation is appropriate after the second miscarriage and that cytogenetic evaluation is necessary for an accurate approach to elucidate the causes of RPL. Moreover, familial adverse reproductive backgrounds have an impact of being carrier of chromosome abnormalities and a larger study is mandatory to define reproductive characteristics of carriers.


How to cite this article:
Frikha R, Turki F, Abdelmoula N, Rebai T. Cytogenetic screening in couples with recurrent pregnancy loss: A single-center study and review of literature.J Hum Reprod Sci 2021;14:191-195


How to cite this URL:
Frikha R, Turki F, Abdelmoula N, Rebai T. Cytogenetic screening in couples with recurrent pregnancy loss: A single-center study and review of literature. J Hum Reprod Sci [serial online] 2021 [cited 2021 Sep 28 ];14:191-195
Available from: https://www.jhrsonline.org/article.asp?issn=0974-1208;year=2021;volume=14;issue=2;spage=191;epage=195;aulast=Frikha;type=0