|Year : 2020 | Volume
| Issue : 4 | Page : 340-348
Chromosomal Aberrations in 224 Couples with Recurrent Pregnancy Loss
Ghada Mohamed Elhady, Soha Kholeif, Nahla Nazmy
Human Genetics Department, Medical Research Institute, Alexandria University, Alexandria, Egypt
|Date of Submission||04-Feb-2020|
|Date of Decision||04-Jun-2020|
|Date of Acceptance||16-Oct-2020|
|Date of Web Publication||28-Dec-2020|
Dr. Ghada Mohamed Elhady
Medical Research Institute, Alexandria University, Mohammed Farid Street, Bolkly, Alexandria
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Background: Recurrent pregnancy loss (RPL) is a major reproductive health issue, affecting 2%–5% of couples. Genetic factors, mainly chromosomal abnormalities, are the most common cause of early miscarriage accounting for 50%–60% of first trimester abortion. Aim: To estimate the prevalence and nature of chromosomal anomalies in couples with recurrent miscarriage. Patients and Methods: This study included 224 couples with a history of 2 or more abortions. Both partners were karyotyped as part of the primary investigation. Cytogenetic analysis was carried out using the standard method. Results: A total of 224 couples with a history of two or more recurrent abortions were enrolled in this study. Chromosomal abnormalities were detected in 26 couples (11.6%) and 28 individuals (6.25%). We found a structural chromosome abnormality in 17/28 patients (60.7%); 12 patients had a reciprocal translocation (42.9%) including one patient with an additional inversion of the Y chromosome, 4 (14.3%) had a Robertsonian translocation, and one patient (3.6%) carried a paracentric inversion of chromosome 2. Numerical chromosome aberrations were detected in 5 patients; three patients (10.7%) with sex chromosome abnormalities and two (7.1%) with a marker chromosome. Six patients (21.4%) showed a heteromorphic variant involving chromosome 9. Conclusion: The prevalence of chromosomal abnormalities in couples with RPL is within the range reported worldwide. Cytogenetic analysis should become an integral part of the investigations of couples with at least two pregnancy losses of undetermined etiology.
Keywords: Chromosomal abnormalities, cytogenetic analysis, recurrent pregnancy loss
|How to cite this article:|
Elhady GM, Kholeif S, Nazmy N. Chromosomal Aberrations in 224 Couples with Recurrent Pregnancy Loss. J Hum Reprod Sci 2020;13:340-8
| Background|| |
Recurrent pregnancy loss (RPL), defined as two or more clinical, nonnecessarily consecutive, pregnancy losses, is considered a major reproductive health issue, affecting 2%–5% of couples. Established etiological factors include genetic abnormalities, endocrine anomalies, anatomical causes, immune factors, inherited thrombophilic disorders, infective agents, lifestyle and environmental factors., Spontaneous abortion is defined as the loss of a clinical pregnancy prior to 20 completed weeks of gestation, or, the loss of an embryo/fetus of <400 g, in case the gestational age is unknown. It is a relatively common event, occurring in 15%–25% of pregnancies, and rises in prevalence with increase in maternal age.
Genetic causes, mainly chromosomal abnormalities, are the most frequent etiological factor of early miscarriage, accounting for 50%–60% of first trimester abortions. Fetal aneuploidy, in which the fetus has an extra or missing autosome or sex chromosome, is the most important cause of miscarriage before 10 weeks gestation. The majority of human aneuploidies arise from errors in the first meiotic division of the oocyte.
RPL may occur if one partner carries a balanced reciprocal translocation, a robertsonian translocation, or an inversion. A reciprocal translocation involves the exchange of two-terminal segments of nonhomologous chromosomes, a robertsonian translocation results from the centric fusion of two acrocentric chromosomes, while an inversion involves a change in the orientation of a DNA segment within a chromosome. Due to abnormal segregation at meiosis, carriers of balanced translocations are at an increased risk not only for RPL, but also for the birth of a disabled child. Inversions suppress recombination within the inverted sequence in heterozygotes, which can directly disrupt coding sequences or alter gene expression of adjacent genes or predispose to other rearrangements, mainly copy number alterations and translocations. Balanced inversion carriers experience decreased fertility, higher rates of miscarriage, and have children with multiple congenital anomalies.
Therefore, identifying couples with a chromosomal anomaly would help in providing proper genetic counseling, including prenatal and preimplantation genetic diagnosis (PGD). The study was carried out with the aim of estimating the prevalence and nature of chromosomal anomalies in couples with recurrent miscarriage.
| Patients and Methods|| |
The research was reviewed and approved by the Ethics Committee (IORG#: IORG0008812). The minimal sample size was calculated based on a study aimed to detect chromosome abnormalities in couples with RPL and to compare our results with those reported previously. Ghazaey et al. (2015) reported that about 11.7% of couples were carriers of chromosomal aberrations. Based on their study, and assuming that 400,000 couples had a history of RPL, 15% out of them had chromosomal aberrations, a minimum sample size of 196 couples with a history of RPL is the enough required sample for estimation of prevalence (cross-sectional) study (Killeen, 2005) (Daniel, 1991), with a significance level of 95% (accepted alpha error of 0.05) and ±5% confidence interval (5% Absolute precision). Sample size per group does not need to be increased to control for attrition bias (Pannucci & Wilkins, 2010).,,, This study included 224 couples (448 individuals) with a history of 2 or more abortions, recruited from Human Genetics clinic from November 2015 to October 2019. Informed consents were obtained from all participants after explanation of the purpose of the study. Couples where the female partner reported history of systemic diseases or thromboembolic disorders were excluded from the study. Both partners were karyotyped as part of the primary investigation. Cytogenetic analysis was performed on peripheral blood lymphocytes incubated for 72 h in media enriched with fetal bovine serum and phytohemagglutinin. Twenty-five metaphases were analyzed following Giemsa trypsin banding at 550 band level. Mosaicism was confirmed if a second cell line was present in more than 5% of cells scored. C-banding was used to confirm the presence of inversion or additional heterochromatin in cases of suspected chromosomal heteromorphy.
Statistical analysis of the data
The sample size was calculated according to Charan and Biswas (2013). Data were fed to the computer and analyzed using IBM SPSS software package version 20.0. (Armonk, NY: IBM Corp). Qualitative data were described using number and percent. Quantitative data were described using range (minimum and maximum), mean, standard deviation, median and interquartile range (IQR). Chi-square test for categorical variables, to compare between different groups Fisher's Exact correction for chi-square when more than 20% of the cells have expected count less than 5. Student t-test for normally distributed quantitative variables, to compare between two studied groups. F-test (ANOVA) for normally distributed quantitative variables, to compare between more than two groups. Mann Whitney test for abnormally distributed quantitative variables, to compare between two studied groups.
| Results|| |
A total of 224 couples with a history of two or more recurrent abortions were enrolled in this study. The mean age of female partners was 28.3 years (range: 16–49 years), whereas the mean age of male partners was 34 years (range: 23–65 years). The number of previous abortions varied from 2 to 16 abortions/couple with a mean of 3.9. We observed no increase in number of abortions with advanced maternal age (P = 0.477), as shown in [Table 1].
Consanguineous mating was observed in 123 couples (54.9%). The frequency was higher among couples with normal karyotypes (56.1%) compared to couples with chromosomal aberrations (46.2%), the difference was not statistically significant (P = 0.34). We detected chromosomal abnormalities in 26 couples (11.6%) and 28 individuals (6.25%). We found a structural chromosome abnormality in 17/28 patients (60.7%); 12 patients had a reciprocal translocation (42.9%) including one patient with an additional inversion of the Y chromosome, 4 (14.3%) had a Robertsonian translocation, and one patient (3.6%) carried a paracentric inversion of chromosome 2. Numerical chromosome aberrations were detected in five patients; three patients (10.7%) with sex chromosome abnormalities and two (7.1%) with a marker chromosome. Six patients (21.4%) showed a heteromorphic variant involving chromosome 9 [Table 2]. Identical chromosomal anomalies were present in both partners in 2 couples; one couple showed a balanced translocation between chromosomes 11 and 22 while the second carried an inversion of the heterochromatic region of chromosome 9. Both couples were consanguineous.
|Table 2: Distribution of the chromosomal abnormalities in affected couples|
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Chromosomal aberrations were more frequent in females (16 patients [7.14%]) compared to males (12 patients [5.35%]), the difference was not statistically significant (P = 0.435).
RPL alone as a presenting feature was more common among couples with chromosomal abnormalities (65.4%) than among couples with a normal karyotype (51.5%), however, this difference was not statistically significant (P = 0.183). The frequency of the presence of a healthy child was higher among couples who had normal karyotypes (33.8%) compared to couples with chromosomal aberrations (23.1%), the difference did not reach statistical significance (P = 0.271). The reproductive outcome of the studied couples is presented in [Table 3] and [Table 4]. [Figure 1],[Figure 2],[Figure 3] show the karyotypes of carriers of balanced rearrangements with chromosomally abnormal offspring.
|Figure 1: (a): Karyotype of female with 46,XX, t(3;7)(p26;p15), (b): Karyotype of offspring with 46,XY, der(3)t(3;7)(p26;p15)mat, (c): Pachytene diagram of the t(3;7)(p26;p15)|
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|Figure 2: (a): Karyotype of female with 46,XX, t(11;22)(q23;q11.2), (b) Karyotype of offspring with 46,XX, t(11;22)(q23;q11.2)mat/pat, (c) Pachytene diagram of the t(11;22)(q23;q11.2)|
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|Figure 3: (a): Karyotype of female with 46,XX, inv(2)(p11.2p23), (b): Karyotype of offspring with 46,XY, rec(2)dup(2)(p15)inv(2)(p11.2p23)mat, (c): Schematic diagram of partial karyogram showing the paracentric inversion chromosome 2 with her offspring|
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|Table 3: Distribution of the studied couples according to reproductive outcome|
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In the present study, more than 50% of couples with a chromosomal anomaly experienced 4 or more abortions, however, this percentage did not reach a statistically significant level (P = 0.241) when compared to couples with normal chromosome complement [Table 5].
|Table 5: Distribution of the studied couples according to number of abortions|
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| Discussion|| |
RPL remains a reproductive challenge both for the clinician and the patient. Carriers of a balanced chromosomal abnormality are at higher risk of generating abnormal gametes leading to stillbirth, recurrent abortions, and the birth of dysmorphic/mentally handicapped infants. Hence, detecting a cytogenetic defect in case of miscarriage may play a significant role in the management of couples with RPL. In the current study, the prevalence of chromosomal aberrations among couples with RPL was 11.6%, which goes in agreement with both international and national studies,, [Table 6]. The discrepancies between various studies may be attributed to differences in sample size, inclusion criteria, and techniques of cytogenetic studies.
|Table 6: Comparison of the frequency of chromosomal aberrations in the present study to the literature|
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The incidence of chromosomal abnormalities was higher among females (7.14%) compared to males (5.35%); some studies reported such difference to be statistically significant, whereas others, including the current study, did not find the difference to be of statistical significance (P = 0.435)., This higher female frequency may be explained by the fact that chromosomal anomalies compatible with fertility in females may be combined with sterility in males., Males with chromosomal aberrations were suggested to have lower fertility rate due to poor spermatic motility, abnormal seminal profile with azoospermia or severe oligoasthenoteratozoospermia.
In the present study, more than 50% of couples with a chromosomal abnormality reported 4 or more abortions; however, no significant difference was detected in the number of abortions experienced by couples with a chromosomal abnormality compared to those with normal karyotypes (P = 0.241), which goes in agreement with other published reports.,
Consanguineous coupling in Egypt is still high, representing 30% of all mating. In the current study, nearly 55% of couples were consanguineous. The frequency of consanguineous mating in the present study was higher among couples with normal karyotype (56.1%) compared to couples with chromosomal anomalies (46.2%), however the difference was not statistically significant (P = 0.34). Identical chromosomal abnormalities were detected in both partners in 2 consanguineous couples; one with a translocation involving chromosomes 11 and 22, and the other couple had an inversion of the heterochromatin of chromosome 9. This rare event where both partners carry the same chromosomal anomaly has been reported in consanguineous Indian couples. The consanguineous couple where both partners carried the same 11; 22 translocation had two living children. Both children were carriers of the familial translocation, one child had polydactyly and polycystic kidney, and the other was normal. The occurrence of clinical expressions in a balanced translocation carrier may be due to physical interruption of genes or a disturbance in their regulatory environment.
In the present study, structural anomalies were 4 times more frequent than numerical aberrations, which goes in agreement with previous reports., Reciprocal translocations were the most commonly identified balanced chromosomal aberrations in couples with RPL, in accord with previous studies.,,, If one partner of a couple carries a balanced chromosomal translocation, the probability of miscarriage is nearly doubled.
Even though carriers of balanced chromosomal rearrangements commonly have a normal phenotype, the probability of generating unbalanced gametes is significant due to complex segregation modes through meiosis. In reciprocal translocation carriers, a quadrivalent arrangement is created at meiosis I via pairing of translocated chromosomes and the two corresponding normal chromosomes. This structure usually undertakes one of three modes of separation: 2:2 (segregation of two chromosomes to one cell and two chromosomes to the other), 3:1 (segregation of three chromosomes to one cell and one to the other) or 4:0 (segregation of all chromosomes of the quadrivalent to one cell and none to the other). Within the 2:2 mode of segregation, chromosomal disjunctions might be alternate, adjacent 1, or adjacent 2. Alternate segregation represents the sole segregation pattern producing gametes with balanced genetic counters: one bearing normal chromosomes while the other carries the balanced translocated chromosomes. Other segregation models will create unbalanced gametes leading to apparent infertility, recurrent abortion, or birth of a phenotypically abnormal offspring with mental retardation or other congenital defects.
In the present study, both adjacent 1 and alternate segregation were observed in the offspring of the carriers of the t(3;7) and t(11;22) respectively. Couples with balanced reciprocal translocation have a 50% risk of RPL and a 20% possibility of having offspring with unbalanced chromosomal rearrangements. The production of unbalanced, balanced, and normal gametes depends on the breakpoints and the chromosomes implicated. The greater imbalance will most probably result in miscarriages, while the slight or less significant imbalance will raise the possibility of having children with unbalanced karyotype. Balanced chromosomal translocations might additionally result in sequence rearrangements of the functional genes that could cause reproductive errors accompanied by RPL.
Robertsonian translocations were less frequently encountered than reciprocal translocations, which agrees with published reports.,,, Robertsonian translocations carry reproductive risks that are dependent on the chromosomes involved and the sex of the carrier. For carriers of the most common Robertsonian translocation der(13;14), the risk for miscarriage is approximately 15%. At meiosis, segregation of trivalent structure may result in nullisomic or disomic gametes for one of the chromosomes involved in the rearrangement and consequently to a zygote with trisomy or monosomy in addition to zygotes with normal chromosome complement or carrying the balanced rearrangement. Zygotes with monosomy are not compatible with life, and most translocation trisomy conceptuses are expected to result in first trimester loss or earlier; however, some survive beyond the second trimester and to term. The risk for trisomy 13 in a carrier of der (13;14) does not exceed 0.4%.
Inversions, both pericentric and paracentric, have been reported in cases with RPL with a frequency lower than Robertsonian translocations,,, as observed in the present study. The risk of pregnancy loss in carriers of a chromosome inversion is not known. The couple with a paracentric inversion of chromosome 2, had 8 abortions, a child with a recombinant karyotype exhibiting multiple congenital anomalies as well as a child with normal chromosome constitution. Hypothetically, heterozygous carriers of paracentric inversions do not generate viable unbalanced offspring. During meiosis, the occurrence of crossing-over event(s), within the inversion loop of affected segments, yields one dicentric and one acentric recombinant chromosome, which are both lethal. However, numerous examples of viable recombinant progeny have been reported., A number of mechanisms explaining the meiotic creation of recombinant stable chromosomes with duplication and/or deletion have been proposed, including unequal crossover, breakage and reunion of sister chromatids, the abnormal process of U-loop recombination and breakage of dicentric recombinants. We propose an unusual mechanism, involving breakage and unequal reunion of sister chromatids within the inversion loop, to explain the structure of our patient's recombinant chromosome.
Polymorphic variants including inversion of chromosome 9 and 9qh+, have been observed in the current study in agreement with previous reports., Heterochromatic polymorphisms, have been implicated in mitotic instability and a tendency towards an increased risk for aneuploidy.
Genetic counseling is preferably offered before subsequent pregnancy; hence, all choices ought to be discussed, and optimum planning assumed. When a couple presents with RPL, detailed family history should be acquired, as this may present clues about familial chromosomal rearrangement. History of congenital anomalies, infertility, mental retardation, spontaneous miscarriage, or perinatal mortality is substantial since each is characteristic of chromosomal anomalies.
Genetic counseling is vital when a structural genetic factor is recognized as there is a risk of having a child with an unbalanced karyotype. When one of the partners carries a structural genetic abnormality, prenatal diagnosis (through amniocentesis, or chorionic villus sampling)/PGD are possible tools to detect the genetic anomaly in the offspring.
| Conclusion|| |
The prevalence of chromosomal abnormalities in Egyptian couples with RPL is within the range reported worldwide. Cytogenetic analysis should become an integral part of the investigations of couples with at least two pregnancy losses of undetermined etiology. Genetic counseling is crucial in the management of couples with RPL. Chromosome abnormalities in couples with repeated abortions are a strong indication for prenatal/PGD, helping a precise reproductive decision considering future pregnancies.
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Conflicts of interest
There are no conflicts of interest.
| References|| |
Practice Committee of the American Society for Reproductive Medicine. Definitions of infertility and recurrent pregnancy loss. Fertil Steril 2008;89:1603.
Practice Committee of the American Society for Reproductive Medicine. Evaluation and treatment of recurrent pregnancy loss: A committee opinion. Fertil Steril 2012;98:1103-11.
Garrido-Gimenez C, Alijotas-Reig J. Recurrent miscarriage: Causes, evaluation and management. Postgrad Med J 2015;91:151-62.
El Hachem H, Crepaux V, May-Panloup P, Descamps P, Legendre G, Bouet PE. Recurrent pregnancy loss: Current perspectives. Int J Womens Health 2017;9:331-45.
Zegers-Hochschild F, Adamson GD, de Mouzon J, Ishihara O, Mansour R, Nygren K, et al
. The International Committee for Monitoring Assisted Reproductive Technology (ICMART) and the World Health Organization (WHO) Revised Glossary on ART Terminology, 2009. Hum Reprod 2009;24:2683-7.
Jacobs PA, Hassold TJ. Chromosome abnormalities: Origin and etiology in abortions and livebirths. In: Vogel F, Sperling K, eds. Proc 7th Int Congr, Berlin,1986. Heidelberg: Springer Verlag1987:233-44.
Herbert M, Kalleas D, Cooney D, Lamb M, Lister L. Meiosis and maternal aging: Insights from aneuploid oocytes and trisomy births. Cold Spring Harb Perspect Biol 2015;7:a017970.
Boué A, Gallano P. A collaborative study of the segregation of inherited chromosome structural rearrangements in 1356 prenatal diagnoses. Prenat Diagn 1984;4:45-67.
Puig M, Casillas S, Villatoro S, Cáceres M. Human inversions and their functional consequences. Brief Funct Genomics 2015;14:369-79.
Feuk L. Inversion variants in the human genome: Role in disease and genome architecture. Genome Med 2010;2:11.
Carp H, Feldman B, Oelsner G, Schiff E. Parental karyotype and subsequent live births in recurrent miscarriage. Fertil Steril 2004;81:1296-301.
Daniel WW. A foundation for analysis in the health sciences. Biostatistics. Toronto, John Wiley & Sons; 1991. p. 209-15.
Ghazaey S, Keify F, Mirzaei F, Maleki M, Tootian S, Ahadian M, et al
. Chromosomal analysis of couples with repeated spontaneous abortions in Northeastern Iran. Int J Fertil Steril 2015;9:47-54.
Killeen PR. An alternative to null-hypothesis significance tests. Psychological Science 2005;16:345-53.
Pannucci CJ, Wilkins EG. Identifying and avoiding bias in research. Plastic and Reconstructive Surgery 2010;126:619-25.
McGowan-Jordan J. An International System for Human Cytogenomic Nomenclature (2016): Recommendations of the International Standing Committee on Human Cytogenomic Nomenclature Including New Sequence-based Cytogenetic Nomenclature Developed in Collaboration with the Human Genome Variation Society (HGVS) Sequence Variant Description Working Group, Karger; 2016.
Zneimer SM. Cytogenetic Laboratory Management: Chromosomal, FISH and Microarray-Based Best Practices and Procedures. New Jersey John Wiley and Sons; 2016.
Charan J, Biswas T. How to calculate sample size for different study designs in medical research? Indian J Psychol Med 2013;35:121-6. doi:10.4103/0253-7176.116232.
] [Full text]
Nazmy NA. Cytogenetic studies of couples with reproductive failure in Alexandria, Egypt. J Egypt Public Health Assoc 2008;83:255-71.
Gaboon NE, Mohamed AR, Elsayed SM, Zaki OK, Elsayed MA. Structural chromosomal abnormalities in couples with recurrent abortion in Egypt. Turk J Med Sci 2015;45:208-13.
De Braekeleer M, Dao TN. Cytogenetic studies in couples experiencing repeated pregnancy losses. Hum Reprod 1990;5:519-28.
Tharapel AT, Tharapel SA, Bannerman RM. Recurrent pregnancy losses and parental chromosome abnormalities: A review. Br J Obstet Gynaecol 1985;92:899-914.
Awartani KA, Al Shabibi MS. Description of cytogenetic abnormalities and the pregnancy outcomes of couples with recurrent pregnancy loss in a tertiary-care Center in Saudi Arabia. Saudi Med J 2018;39:239-42.
Eltayeb SM, Ambusaidi SK, Gowri V, Alghafri WM. Etiological profile of Omani women with recurrent pregnancy loss. Saudi Med J 2014;35:757-60.
Houmaid H, El Bekkay C, Nassereddine S, Talbi H, Amehdare L, Hilali A. Chromosomal abnormalities in 238 couples with recurrent miscarriages in Morocco. Open J Gen 2018;8:15-22.
Boue A, Boue J, Gropp A. Cytogenetics of pregnancy wastage. In: Advances in Human Genetics. 14th
ed. Boston: Springer; 1985. p. 1-57.
Stephenson MD, Awartani KA, Robinson WP. Cytogenetic analysis of miscarriages from couples with recurrent miscarriage: A case-control study. Hum Reprod 2002;17:446-51.
Tunç E, Tanrıverdi N, Demirhan O, Süleymanova D, Çetinel N. Chromosomal analyses of 1510 couples who have experienced recurrent spontaneous abortions. Reprod Biomed Online 2016;32:414-9.
Pal AK, Ambulkar PS, Waghmare JE, Wankhede V, Shende MR, Tarnekar AM. Chromosomal aberrations in couples with pregnancy loss: A retrospective study. J Hum Reprod Sci 2018;11:247-53.
] [Full text]
Atia TA, Mourad SE. Cytogenetic study in couples with recurrent miscarriage. Egypt J Hosp Med 2007;26:46-54.
Ocak Z, Özlü T, Ozyurt O. Association of recurrent pregnancy loss with chromosomal abnormalities and hereditary thrombophilias. Afr Health Sci 2013;13:447-52.
Chandley AC, Edmond P, Christie S, Gowans L, Fletcher J, Frackiewicz A, et al
. Cytogenetics and infertility in man. I. Karyotype and seminal analysis: Results of a five-year survey of men attending a subfertility clinic. Ann Hum Gen 1975;39:231-54.
van den Berg MM, van Maarle MC, van Wely M, Goddijn M. Genetics of early miscarriage. Biochim Biophys Acta 2012;1822:1951-9.
Dubey S, Chowdhury M, Prahlad B, Kumar V, Mathur R, Hamilton S, et al
. Cytogenetic causes for recurrent spontaneous abortions-an experience of 742 couples (1484 cases). Indian Journal of Human Genetics 2005;11:94-8.
Shawky RM, El-Awady MY, Elsayed SM, Hamadan GE. Consanguineous matings among Egyptian population. Egypt J Med Hum Gen 2011;12:157-63.
Sanyal D, Bhairi V, S Kadandale J. Practice of consanguinity and unusual cases of inherited familial chromosome abnormalities: A case report. Int J Mol Cell Med 2016;5:57-63.
Sobreira NL, Gnanakkan V, Walsh M, Marosy B, Wohler E, Thomas G, et al
. Characterization of complex chromosomal rearrangements by targeted capture and next-generation sequencing. Genome Res 2011;21:1720-7.
Kavalier F. Investigation of recurrent miscarriages. BMJ 2005;331:121-2.
Lim CK, Cho JW, Song IO, Kang IS, Yoon YD, Jun JH. Estimation of chromosomal imbalances in preimplantation embryos from preimplantation genetic diagnosis cycles of reciprocal translocations with or without acrocentric chromosomes. Fertil Steril 2008;90:2144-51.
Zhang Y, Zhu S, Wu J, Liu S, Sun X. Quadrivalent asymmetry in reciprocal translocation carriers predicts meiotic segregation patterns in cleavage stage embryos. Reprod Biomed Online 2014;29:490-8.
Farcas S, Belengeanu V, Popa C, Stoicanescu D, Stoian M, Veliscu M, et al
. Role of chromosomal translocations in recurrent spontaneous abortion. Timisoara Med J 2007;2:117-21.
Harris DJ, Hankins L, Begleiter ML. Reproductive risk of t (13q14q) carriers: Case report and review. Am J Med Genet 1979;3:175-81.
Scriven PN, Flinter FA, Braude PR, Ogilvie CM. Robertsonian translocations--reproductive risks and indications for preimplantation genetic diagnosis. Hum Reprod 2001;16:2267-73.
Phelan MC, Stevenson RE, Anderson EV Jr. Recombinant chromosome 9 possibly derived from breakage and reunion of sister chromatids within a paracentric inversion loop. Am J Med Genet 1993;46:304-8.
Lefort G, Blanchet P, Belgrade N, Rivier F, Chaze AM, Sarda P, et al
. Stable dicentric duplication-deficiency chromosome 14 resulting from crossing-over within a maternal paracentric inversion. Am J Med Gen 2002;113:333-8.
Yang SP, Bidichandani SI, Figuera LE, Juyal RC, Saxon PJ, Baldini A, et al
. Molecular analysis of deletion (17) (p11. 2p11. 2) in a family segregating a 17p paracentric inversion: Implications for carriers of paracentric inversions. Am J Hum Gen 1997;60:1184-93.
Mitchell JJ, Vekemans M, Luscombe S, Hayden M, Weber B, Richter A, et al
. U-type exchange in a paracentric inversion as a possible mechanism of origin of an inverted tandem duplication of chromosome 8. Am J Med Gen 1994;49:384-7.
Madan K. Paracentric inversions: A review. Hum Genet 1995;96:503-15.
Fischer J, Colls P, Escudero T, Munné S. Preimplantation genetic diagnosis (PGD) improves pregnancy outcome for translocation carriers with a history of recurrent losses. Fertil Steril 2010;94:283-9.
[Figure 1], [Figure 2], [Figure 3]
[Table 1], [Table 2], [Table 3], [Table 4], [Table 5], [Table 6]