|Year : 2020 | Volume
| Issue : 3 | Page : 174-183
Does the use of mobile phone affect male fertility? A mini-review
Chidiebere Emmanuel Okechukwu
Department of Public Health and Infectious Diseases, Sapienza University of Rome, Rome, Italy
|Date of Submission||11-Sep-2019|
|Date of Decision||01-Nov-2019|
|Date of Acceptance||10-Aug-2020|
|Date of Web Publication||27-Oct-2020|
Dr. Chidiebere Emmanuel Okechukwu
Department of Public Health and Infectious Diseases, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Presently, there is a rise in the use of mobile phones, laptops, and wireless internet technologies such as Wi-Fi and 5G routers/modems across the globe; these devices emit a considerable amount of electromagnetic radiation (EMR) which could interact with the male reproductive system either by thermal or nonthermal mechanisms. The aim of this review was to examine the effects of mobile phone use on male fertility. Related studies that reported on the effects of EMR from mobile phones on male fertility from 2003 to 2020 were evaluated. PubMed database was used. The Medical Subject Heading system was used to extract relevant research studies from PubMed. Based on the outcomes of both human and animal studies analyzed in this review, animal and human spermatozoa exposed to EMR emitted by mobile phones had reduced motility, structural anomalies, and increased oxidative stress due to overproduction of reactive oxygen species. Scrotal hyperthermia and increased oxidative stress might be the key mechanisms through which EMR affects male fertility. However, these negative effects appear to be associated with the duration of mobile phone use.
Keywords: Electromagnetic fields, male reproductive health, mobile phone, wireless technology
|How to cite this article:|
Okechukwu CE. Does the use of mobile phone affect male fertility? A mini-review. J Hum Reprod Sci 2020;13:174-83
| Introduction|| |
Presently, there is a rise in the use of mobile phones, laptops, and wireless internet technologies such as Wi-Fi and 5G routers/modems across the globe; these devices emit a considerable amount of electromagnetic radiation (EMR) which could interact with the male reproductive system either by thermal or nonthermal mechanisms. EMR might alter male reproductive-endocrine mechanisms by affecting testicular functions necessary for testosterone and sperm production. Most studies conducted in this field were based on animal experimentation. The human testis is very subtle to radiation and heat. Short-term exposure of male Wistar rats to mobile phone radiation caused a slight decrease in serum testosterone levels and testicular weight.
Samples of human ejaculated semen exposed to EMR emitted by a cell phone showed a significant reduction in sperm motility and viability and increase in reactive oxygen species (ROS) level; this was achieved by the collection of semen samples from 23 healthy controls and 9 patients at an infertility clinic, where semen samples were collected through masturbation after an abstinence period of 48–72 h and allowed to liquefy completely for 15–30 min at 37°C. Ensuing liquefaction, the semen samples were categorized into two groups: control group (sample not exposed to mobile phone radiation) and exposed group (sample exposed to cell phone radiation). Semen samples in the exposed group were exposed to EMR emitted from a mobile phone in talk mode (the mobile phone device was Sony Ericsson w300i; the service provider was AT and T; Global System for Mobile communications network; 850 MHz frequency; maximum power <1 W; specific absorption rate 1.46 W/kg), the distance between the cell phone antenna and each specimen was kept at 2.5 cm, and the duration of exposure was 60 min, however the power density of the mobile phone was observed during basal condition (no cell phone radiation) and experimental condition (cell phone in talk mode) in the laboratory throughout the experiment. Power density in the control state was 0.01–0.1 μW/cm2, while power density in the experimental condition (during cell phone in talk mode and at 2.5 cm from cell phone antenna) was 1–40 μW/cm2, and the frequency discharged by the cell phone was checked with radiofrequency (RF) spectrum analyzer (Tektronix, Beaverton). Both groups were kept at room temperature to avoid the effect of temperature on ROS formation and semen parameters. After exposing the semen in the exposed group to mobile phone radiation, the semen samples from both groups (control and exposed) were examined for sperm concentration, motility, and viability. ROS was measured in the exposed and control groups after 1 h through chemiluminescence assay using luminol.
There was a statistically significant decrease in the laboratory values of mean sperm count, morphology, motility, and quality/viability, among four different mobile phone user groups, exposed to mobile phone EMR daily. EMR induced a significant genotoxic effect on epididymal spermatozoa. Normal sperm samples exposed to a laptop computer connected to Wi-Fi for 4 h exhibited a significant reduction in sperm motility and an upsurge in sperm deoxyribonucleic acid (DNA) disintegration. There was a significant association between exposure to RF electromagnetic field (EMF) with higher rate of childlessness among military men working for the Royal Norwegian Navy.
RF-EMF in the power density and frequency range of cell phones increases mitochondrial ROS production in human spermatozoa and DNA damage. Long-term exposure to EMR, both high and low frequency, might affect the male germ cell in humans. However, exposure to EMR altered pituitary function in rats. Constant exposure to EMR affected the Leydig and Sertoli cells More Details in male rats, thereby altering male fertility., There was an increase in free radicals and subsequently oxidative stress, decrease in sperm concentration, and decrease in motility and viability among men that were exposed to EMR. Long-term exposure to mobile phone EMR may cause reductions in serum testosterone levels. EMR alters normal reproductive processes in male rats; the same might occur in male humans exposed to RF-EMF over a long period of time., The internal circuits of laptops and mobile phones used by male individuals regularly generates EMR [Figure 1]. The aim of this review was to examine the effects of mobile phone use on male fertility.
|Figure 1: Electromagnetic radiation spectrum (Copyright 1999, the Radiation Research Society)|
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| Materials and Methods|| |
Related studies that reported on the effects of EMR from mobile phones on male fertility from 2003 to 2020 were evaluated. PubMed database was used. The Medical Subject Heading system was used to extract relevant research studies from PubMed using the following keywords: mobile phone, cellular phone, laptop computers, wireless technology, male reproductive health, electromagnetic fields, and radiowaves.
Recent findings from human experimentation
Long-term exposure to EMR emitted by mobile phones and Wi-Fi devices, might play a role in male infertility because of an increased production of ROS in exposed cells [Figure 2], [Figure 3], [Figure 4], [Figure 5].
|Figure 2: Possible effects of electromagnetic radiation exposure on testicular function|
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|Figure 3: Biological effects of radiofrequency-electromagnetic field exposure on male fertility. (Kesari KK, Kumar S, Nirala J, Siddiqui MH, Behari J. Biophysical evaluation of radiofrequency electromagnetic field effects on male reproductive pattern. Cell Biochem Biophys 2013;65:85-96.)|
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|Figure 4: Effects of electromagnetic radiation exposure on testicular cells. (Agarwal A, Singh A, Hamada A, Kesari K. Cell phones and male infertility: A review of recent innovations in technology and consequences. Int Braz J Urol 2011;37:432-54.)|
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|Figure 5: Possible effects of radiofrequency-electromagnetic field on the mitochondrial electron transport chain of male spermatozoa. (Houston BJ, Nixon B, King BV, De Iuliis GN, Aitken RJ. The effects of radiofrequency electromagnetic radiation on sperm function. Reproduction 2016;152:R263-76.)|
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RF-EMF increases ROS production by augmenting the action of nicotinamide adenine dinucleotide oxidase in the human cell membrane. An examination of 468 men attending an infertility clinic from 1993 to 2007 with the aim of assessing if there is a combined effect of varicocele and carrying mobile phones in trouser pockets on semen and hormonal parameters was conducted during clinical checkup and the patients were questioned on mobile phone use. Their semen samples, serum testosterone, luteinizing hormone (LH), and follicle-stimulating hormone (FSH) were analyzed, and the results of the analysis showed that there was a consequence of keeping mobile phones in trouser pockets on testicular function. Mobile phone storage in trouser pockets presented a statistically significant decrease in the percentage of normal sperm morphology and LH (both P < 0.001), however varicocele and keeping mobile phone in trouser pocket did not show a combined effect (P = 0.76).
Zalata et al. exposed 124 human semen samples to mobile phone EMR, and they observed significant decrease in sperm motility, sperm linear velocity, sperm linearity index, and sperm acrosin activity, moreover there was a significant increase in sperm DNA fragmentation in the exposed semen samples to RF-EMF compared with nonexposed samples.
Zhang et al. examined the mobile phone use data of 794 young men which they obtained from the 2013 Chongqing College students cohort study on male reproductive health, they also extracted the mobile phone use data of 666 and 568 young men from the Chongqing College students cohort study in 2014 and 2015, respectively, they found that the number of time spent talking on the mobile phone was significantly associated with low volume of semen, sperm concentration and total sperm count, and that constant use of mobile phone internet service was linked to reduced sperm concentration and total sperm count in 2013 and reduced semen volume in 2015, these shows that a significant association exist between mobile phone internet use and poor sperm quality, the summary of the outcomes of the cohort studies was that EMR from mobile phone harmfully affect sperm quality by lowering the semen volume, sperm concentration, and sperm count thus affecting male fertility.
Zilberlicht et al. worked on the association between mobile phone use and semen quality. Surveys designed with reference to demographic information on mobile phone use were filled by 106 men referred for semen analysis; the outcomes were analyzed, which showed that talking time of more than 1 h per day was related to a higher percentage of abnormal sperm concentration than speaking <1 h per day (60.9% vs. 35.7%, P < 0.04). The use of cell phone while charging it was associated with a higher percentage of abnormal sperm concentration compared to not using it while charging (66.7% vs. 35.6%, P < 0.02); this might be because while charging a mobile phone, the external power source emits energy and owing to the unceasing supply of energy from the external source, the device transmits at a higher power, without the need for energy saving, which is different when compared to the usual talking mode. Concerning the location of the mobile phone, while it was not in use, 87.6% of the participants stated that the device was constantly held at a distance of <50 cm from the groin (47.5% in trouser pocket, 22.6% in their hands or on their belt, 2.5% in their shirt pocket, and 15% in other place), however the percentage of abnormal sperm concentration was not significant among participants who kept their mobile phones at a distance ≤50 cm from the groin compared with participants who kept their cell phones at a distance >50 cm from the groin (47.1% vs. 11.1%). However, Kilgallon and Simmons observed that men who carried their cell phones in their hip pocket and on their belt had lower sperm motility (49.3% ± 8.2%) than men who did not carry a cell phones or who carried their cell phones phone in other places in the body (55.4% ± 7.4%; P < 0.0001).
Low RF-EMF of 15 Hz with a peak intensity of 8 Gauss, and a square waveform of 50 Hz, at a duration of few hours or less did not affect sperm quality, motility, and fertility. It is very important to minimize the time spent on the mobile phone browsing the Internet or making a phone call in order to minimize the negative effects of cell phone EMR. A summary of the recent findings on the effects of RF-EMF on male fertility is summarized in [Table 1].
|Table 1: Recent findings on the effects of radiofrequency electromagnetic field on male fertility|
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| Discussion|| |
The evidences on the harmful effects of mobile phone EMR on male fecundity are still ambiguous and the biological effects of RF-EMF emitted by these devices on human reproductive system are not fully determined because most of the studies carried out on EMR exposure were mainly conducted on rats. Increased use of mobile phones worldwide has made mobile phone base positions to be sited in houses, schools, and hospitals. This has raised public concerns especially on the latest 5th generation mobile communication technology (5G technology), regarding the safety of inhabitants exposed to radiation from these devices.
Because of the advancement in mobile telecommunication technology, there is a need to conduct more research on the interactions between this latest and popular smartphones and human health. A reduction in the proportion of sperm cells in the semen was associated with the frequency of using mobile phones, however, in a meta-analysis study comprising 1492 samples, constant use of mobile phones was linked to reduced sperm motility. This advocates that mobile phone exposure undesirably affects sperm quality, thus promoting male infertility. Exposure to mobile phone EMR decreases sperm viability, motility, and sperm total antioxidant capacity in rats and results in oxidative stress. EMR exposure caused an injury in the blood–testis barrier and induced immune infertility in male mice. However, there is a need to conduct more investigations to determine the thermal and nonthermal effects of the 5G smartphones RF emissions on cell membrane structures and immune system.
Personal protective measures to reduce electromagnetic radiation
Humans should limit their rate of exposure to RF-EMF-emitting devices including laptop computers, Wi-Fi, and mobile base stations. In order to minimize the exposure to EMR emitted by cell phones, male individuals should avoid keeping their cell phones inside their trouser pocket for a long period of time because of the thermal effect of mobile phone radiation and proximity to the testes, and the use of hand-free materials and gadgets that limit direct contact of cell phones with the body should be encouraged. An example is the Bluetooth earpiece, and the use of shield case or purse should be encouraged by cell phone manufacturers in order to minimize cell phone EMR emission. However, Vitamins C and E and some natural supplements have been shown to be effective in protecting the testis against RF-EMF effects [Table 2].
|Table 2: Supplements that can protect the testes against electromagnetic radiation|
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Limitations of studies that investigated the effects of mobile phone electromagnetic radiation emission on human and animal physiology
According to Kostoff et al., most laboratory studies conducted on humans and animals were not designed to identify the more severe adverse effects of RF-EMF exposure. Numerous experimentations did not include the real-life pulsing and modulation of the 5G mobile technology carrier signal. Many of the experimental studies did not investigate the merged harmful effects of other toxic environmental stimuli with wireless EMR and their mechanism of interactions to diminish male fertility [Figure 6].
|Figure 6: Possible environmental factors that might synergize with electromagnetic radiation in decreasing male fertility. (Sengupta P, Dutta S, Krajewska-Kulak E. The Disappearing Sperms: Analysis of Reports Published Between 1980 and 2015. Am J Mens Health 2017;11:1279-304.)|
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More funded laboratory experimental studies should be conducted to determine the effects of the latest 5G mobile technology devices and equipment (smartphones, Wi-Fi, 5G router, and masts) on male reproductive function with emphasis on the speed, power, latency, and carrier frequencies of the 5G signals and their interactions with other toxic environmental stimuli in disrupting male reproductive-endocrine physiology. Any adverse effect associated with 5G wireless radiation should be well established. Stationing 5G mobile technology equipment in an environment that have been marked for harmful wireless radiation might aggravate the adverse health effects associated with RF-EMF; more studies are needed to determine the synergistic effects of RF-EMF emitted by 5G mobile technology facilities and RF-EMF emitted from other facilities such as power stations for optimum environmental health assessment and protection. However, mobile telecommunication companies and manufactures should endeavor to fund large-scale epidemiological studies with personal dosimeters for precise dose measurement and rate of tissue exposure, by determining the adverse effects of RF-EMF emitted by 4G and 5G mobile technology. This will enable the creation of future plans in order to lessen the risk of adverse health effects from unintentional human harm due to exposure to RF-EMF from mobile telecommunication devices and equipment. There is a need to conduct vast laboratory experimentations in order to investigate and determine the adverse effects of RF-EMF exposure on the hypothalamus most especially gonadotropin-releasing hormone and anterior pituitary gland with respect to FSH, LH, and prolactin secretions. This will contribute immensely in establishing the harmful effects of EMR exposure on male fertility [Figure 7].
|Figure 7: Potential interaction between mobile phone radiofrequency-electromagnetic field, hypothalamus, anterior pituitary gland, and the testis. (Altun G, Deniz ÖG, Yurt KK, Davis D, Kaplan S. Effects of mobile phone exposure on metabolomics in the male and female reproductive systems. Environ Res 2018;167:700-7.)|
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| Conclusion|| |
Based on the outcomes of both human and animal studies examined in this review, animal and human spermatozoa exposed to EMR emitted by mobile phones had reduced motility, structural anomalies, and increased oxidative stress due to the production of ROS. Scrotal hyperthermia and increased oxidative stress might be the key mechanisms by which EMR affects male fertility. However, these negative effects appear to be associated with the duration of mobile phone use.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Okechukwu CE. Effects of mobile phone radiation and exercise on testicular function in male Wistar rats. Niger J Exp Clin Biosci 2018;6:51-8. [Full text]
Agarwal A, Desai NR, Makker K, Varghese A, Mouradi R, Sabanegh E, et al
. Effects of radiofrequency electromagnetic waves (RF-EMW) from cellular phones on human ejaculated semen: Anin vitro
pilot study. Fertil Steril 2009;92:1318-25.
Agarwal A, Deepinder F, Sharma RK, Ranga G, Li J. Effect of cell phone usage on semen analysis in men attending infertility clinic: An observational study. Fertil Steril 2008;89:124-8.
Aitken RJ, Bennetts LE, Sawyer D, Wiklendt AM, King BV. Impact of radio frequency electromagnetic radiation on DNA integrity in the male germline. Int J Androl 2005;28:171-9.
Avendaño C, Mata A, Sanchez Sarmiento CA, Doncel GF. Use of laptop computers connected to internet through Wi-Fi decreases human sperm motility and increases sperm DNA fragmentation. Fertil Steril 2012;97:39-4500.
Baste V, Riise T, Moen BE. Radiofrequency electromagnetic fields; male infertility and sex ratio of offspring. Eur J Epidemiol 2008;23:369-77.
de Iuliis GN, Newey RJ, King BV, Aitken RJ. Mobile phone radiation induces reactive oxygen species production and DNA damage in human spermatozoa in vitro
. PLoS One 2009;4:e6446.
Erogul O, Oztas E, Yildirim I, Kir T, Aydur E, Komesli G, et al
. Effects of electromagnetic radiation from a cellular phone on human sperm motility: Anin vitro
study. Arch Med Res 2006;37:840-3.
Fang HH, Zeng GY, Nie Q, Kang JB, Ren DQ, Zhou JX, et al
. Effects on structure and secretion of pituitary gland in rats after electromagnetic pulse exposure. Zhonghua Yi Xue Za Zhi 2010;90:3231-4.
Fejes I, Závaczki Z, Szöllosi J, Koloszár S, Daru J, Kovács L, et al
. Is there a relationship between cell phone use and semen quality? Arch Androl 2005;51:385-93.
Kesari KK, Behari J. Microwave exposure affecting reproductive system in male rats. Appl Biochem Biotechnol 2010;162:416-28.
La Vignera S, Condorelli RA, Vicari E, D'Agata R, Calogero AE. Effects of the exposure to mobile phones on male reproduction: A review of the literature. J Androl 2012;33:350-6.
Mailankot M, Kunnath AP, Jayalekshmi H, Koduru B, Valsalan R. Radio frequency electromagnetic radiation (RF-EMR) from GSM (0.9/1.8GHz) mobile phones induces oxidative stress and reduces sperm motility in rats. Clinics (Sao Paulo) 2009;64:561-5.
Meo SA, Al-Drees AM, Husain S, Khan MM, Imran MB. Effects of mobile phone radiation on serum testosterone in Wistar albino rats. Saudi Med J 2010;31:869-73
Wang SM, Wang DW, Peng RY, Gao YB, Yang Y, Hu WH, et al
. Effect of electromagnetic pulse irradiation on structure and function of Leydig cells in mice. Zhonghua Nan Ke Xue 2003;9:327-30.
Kesari KK, Agarwal A, Henkel R. Radiations and male fertility. Reprod Biol Endocrinol 2018;16:118.
Yahyazadeh A, Deniz ÖG, Kaplan AA, Altun G, Yurt KK, Davis D. The genomic effects of cell phone exposure on the reproductive system. Environ Res 2018;167:684-93.
Schauer I, Mohamad Al-Ali B. Combined effects of varicocele and cell phones on semen and hormonal parameters. Wien Klin Wochenschr 2018;130:335-40.
Zalata A, El-Samanoudy AZ, Shaalan D, El-Baiomy Y, Mostafa T.In vitro
effect of cell phone radiation on motility, DNA fragmentation and clusterin gene expression in human sperm. Int J Fertil Steril 2015;9:129-36.
Zhang G, Yan H, Chen Q, Liu K, Ling X, Sun L, et al
. Effects of cell phone use on semen parameters: Results from the MARHCS cohort study in Chongqing, China. Environ Int 2016;91:116-21.
Zilberlicht A, Wiener-Megnazi Z, Sheinfeld Y, Grach B, Lahav-Baratz S, Dirnfeld M. Habits of cell phone usage and sperm quality-does it warrant attention? Reprod Biomed Online 2015;31:421-6.
Kilgallon SJ, Simmons LW. Image content influences men's semen quality. Biol Lett 2005;1:253-5.
Darbandi M, Darbandi S, Agarwal A, Henkle R, Sadeghi MR. The effects of exposure to low frequency electromagnetic fields on male fertility. Altern Ther Health Med 2018;24:24-9.
Houston BJ, Nixon B, McEwan KE, Martin JH, King BV, Aitken RJ, et al
. Whole-body exposures to radiofrequency-electromagnetic energy can cause DNA damage in mouse spermatozoa via an oxidative mechanism. Sci Rep 2019;9:17478.
Yu G, Tang Z, Chen H, Chen Z, Wang L, Cao H, et al
. Long-term exposure to 4G smartphone radiofrequency electromagnetic radiation diminished male reproductive potential by directly disrupting Spock3-MMP2-BTB axis in the testes of adult rats. Sci Total Environ 2020;698:133860.
Górski R, Kotwicka M, Skibińska I, Jendraszak M, Wosiński S. Effect of low-frequency electric field screening on motility of human sperm. [published online ahead of print January 27, 2020]. Ann Agric Environ Med. https://doi.org/10.26444/aaem/116019
Gautam R, Singh KV, Nirala J, Murmu NN, Meena R, Rajamani P. Oxidative stress-mediated alterations on sperm parameters in male Wistar rats exposed to 3G mobile phone radiation. Andrologia 2019;51:e13201.
Oh JJ, Byun SS, Lee SE, Choe G, Hong SK. Effect of electromagnetic waves from mobile phones on spermatogenesis in the Era of 4G-LTE. Biomed Res Int 2018;2018:1801798.
Adams JA, Galloway TS, Mondal D, Esteves SC, Mathews F. Effect of mobile telephones on sperm quality: A systematic review and meta-analysis. Environ Int 2014;70:106-12.
Ghanbari M, Mortazavi SB, Khavanin A, Khazaei M. The effects of cell phone waves (900 MHz-GSM Band) on sperm parameters and total antioxidant capacity in rats. Int J Fertil Steril 2013;7:21-8.
Wang XW, Ding GR, Shi CH, Zeng LH, Liu JY, Li J, et al
. Mechanisms involved in the blood-testis barrier increased permeability induced by EMP. Toxicology 2010;276:58-63.
Okechukwu CE. Effects of radiofrequency electromagnetic field exposure on neurophysiology. Adv Hum Biol 2020;10:6-10. [Full text]
Al-Damegh MA. Rat testicular impairment induced by electromagnetic radiation from a conventional cellular telephone and the protective effects of the antioxidants vitamins C and E. Clinics (Sao Paulo) 2012;67:785-92.
Bin-Meferij MM, El-Kott AF. The radioprotective effects of Moringa oleifera
against mobile phone electromagnetic radiation-induced infertility in rats. Int J Clin Exp Med 2015;8:12487-97.
Ma Y, Jia X. Polydatin alleviates radiation-induced testes injury by scavenging ROS and inhibiting apoptosis pathways. Med Sci Monit 2018;24:8993-9.
Ding Z, Li J, Li F, Mephryar MM, Wu S, Zhang C, et al
. Vitamin C and Vitamin E protected B95-8 and Balb/c-3T3 cells from apoptosis induced by intermittent 50Hz ELF-EMF radiation. Iran J Public Health 2017;46:23-34.
Kostoff RN, Heroux P, Aschner M, Tsatsakis A. Adverse health effects of 5G mobile networking technology under real-life conditions. Toxicol Lett 2020;323:35-40.
Pearce JM. Limiting liability with positioning to minimize negative health effects of cellular phone towers. Environ Res 2020;181:108845.
[Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6], [Figure 7]
[Table 1], [Table 2]