Journal of Human Reproductive Science
Home Ahead of Print Current Issue Archives
   Bookmark this page Print this page Email this page Small font sizeDefault font size Increase font size    Users online: 833

ORIGINAL ARTICLE Table of Contents   
Year : 2016  |  Volume : 9  |  Issue : 4  |  Page : 215-222
Role of progenitor cell producing normal vagina by metaplasia in laparoscopic peritoneal vaginoplasty

1 Department of Obstetrics and Gynecology, Seth G S Medical College, Nowrosjee Wadia Maternity Hospital, Parel; Department of Genetic, Kedar Hospital, Mumbai, Maharashtra, India
2 Department of Obstetrics and Gynecology, Seth G S Medical College, Nowrosjee Wadia Maternity Hospital, Parel, India
3 Department of Genetic, Kedar Hospital, Mumbai, Maharashtra, India
4 Genetic Research Centre, National Institute for Research in Reproductive Health, Parel, India

Correspondence Address:
Pravin N Mhatre
9/2nd Floor Mohan Niwas, Keluskar Road, Shivaji Park, Mumbai - 400 028, Maharashtra
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0974-1208.197629

Rights and Permissions

Context: Host of vaginoplasty techniques have been described. None has been successful in developing normal vagina. Laparoscopic peritoneal vaginoplasty (LPV) is performed in Mayer-Rokitansky-Küster-Hauser syndrome (MRKHS) culminating in normal vagina. Aims: This study aims to confirm normal development of neovagina by anatomical and functional parameters of histology, cytology, and ultrasonography (USG) in LPV. To identify peritoneal progenitor cell by OCT4/SOX2 markers. To demonstrate the metaplastic conversion of peritoneum to neovagina and the progenitor cell concentration, distribution pattern. Settings and Design: This is prospective experimental study, conducted at teaching hospital and private hospital. Subjects and Methods: Fifteen women of MRKHS underwent LPV followed by histology, cytology, two-/three-dimensional USG of neovagina. Four women underwent peritoneal biopsy for identification of progenitor cells with OCT4/SOX2 markers. One patient underwent serial biopsies for 4 weeks for histology and progenitor cell immunohistochemistry. Results: Normal vaginal histology and cytology were apparent. USG of neovagina showed normal appearance and blood flow. Two peritoneal samples confirmed the presence of progenitor cells. Serial biopsies demonstrated the epithelial change from single to multilayer with stromal compaction and neoangiogenesis. The progenitor cells concentration and different distribution patterns were described using SOX2/OCT4 markers. Conclusions: We have shown successful peritoneal metaplastic conversion to normal vagina in LPV. The progenitor cell was identified in normal peritoneum using SOX2/OCT4 markers. The progenitor cell concentration and pattern were demonstrated at various stages of neovaginal development.

Print this article  Email this article

  Similar in PUBMED
    Search Pubmed for
    Search in Google Scholar for
  Related articles
   Citation Manager
  Access Statistics
   Reader Comments
   Email Alert *
   Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded163    
    Comments [Add]    
    Cited by others 2    

Recommend this journal