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| ORIGINAL ARTICLE |
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| Year : 2013 |
Volume
: 6 | Issue : 1 | Page
: 23-26 |
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Does centrifugation and semen processing with swim up at 37°C yield sperm with better DNA integrity compared to centrifugation and processing at room temperature?
Deepthi Repalle, Priya Bhave Chittawar, Shilpa Bhandari, Geetanjali Joshi, Mansi Paranjape, Charudutta Joshi
Department of Reproductive Medicine, Mohak Hi-tech Speciality Laparoscopy and Infertility Centre, Sri Aurobindo Institute of Medical Sciences, Indore, Madhya Pradesh, India
Correspondence Address:
Deepthi Repalle
Senior Embryologist, Mohak Hi-tech Specialty Laparoscopy and Infertility center, SAIMS campus, Indore-Ujjain State Highway, M.R.-10, Indore, Madhya Pradesh.452010
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/0974-1208.112375
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Aim: To evaluate whether semen processing at 37°C yield sperm with better DNA integrity compared to centrifugation and processing at room temperature (RT) by swim-up method. Settings: This study was done at tertiary care center attached to Reproductive Medicine Unit and Medical College. Design: Prospective pilot study. Patients: Normozoospermic men (n = 50) undergoing diagnostic semen analysis. Materials and Methods: Normozoospermic samples (World Health Organization, 2010 criteria) after analysis was divided into two aliquots (0.5 mL each); one was processed at 37°C and the other at RT by swim-up method. DNA fragmentation of both samples post wash was calculated by acridine orange method. Statistical Analysis Used: The values of sperm DNA fragmentation were represented as mean and standard error (mean ± SEM) of the mean. Paired t-test was used for calculating the sperm DNA integrity difference between post wash at RT and 37°C. Results: Statistically significant difference was not observed in post wash sperm DNA fragmentation values at 37°C compared to RT. Conclusion: Our data represents that there was no significant difference in sperm DNA fragmentation values of samples processed at 37°C and at RT. Hence, sperm processing at 37°C does not yield sperm with better DNA integrity compared to centrifugation and processing at RT. |
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