Journal of Human Reproductive Science
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Year : 2010  |  Volume : 3  |  Issue : 3  |  Page : 135-138

Effect of body mass index on in vitro fertilization outcomes in women

1 Consultant Endocrinologist, Institute of Reproductive Medicine and Women's Health, Madras Medical Mission Hospital, Chennai, Tamil Nadu, India
2 Consultants in Reproductive Medicine, Institute of Reproductive Medicine and Women's Health, Madras Medical Mission Hospital, Chennai, Tamil Nadu, India

Date of Submission10-Jan-2010
Date of Decision30-Apr-2010
Date of Acceptance27-Aug-2010
Date of Web Publication23-Dec-2010

Correspondence Address:
Anjali Sathya
Consultant Endocrinologist, Institute of Reproductive Medicine and Women's Health, Madras Medical Mission Hospital, Chennai, Tamil Nadu
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Source of Support: None, Conflict of Interest: None

PMID: 21234174

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Background :Obesity has become a major health problem across the world. In women, it is known to cause anovulation, subfecundity, increased risk of fetal anomalies and miscarriage rates. However, in women going for assisted reproduction the effects of obesity on egg quality, embryo quality, clinical pregnancy, live birth rates are controversial. Objectives :To assess the effect of women's body mass index (BMI) on the reproductive outcome of non donor In vitro fertilization (IVF)/Intracytoplasmic sperm injection (ICSI). The effects of BMI on their gonadotrophin levels (day 2 LH, FSH), gonadotrophin dose required for ovarian stimulation, endometrial thickness and oocyte/embryo quality were looked at, after correcting for age and poor ovarian reserve. Materials and Methods : Retrospective study of medical records of 308 women undergoing non donor IVF cycles in a University affiliated teaching hospital. They were classified into three groups: normal weight (BMI<25 kg/m 2 ), overweight (BMI>25 <30 kg/m 2 ) and obese (BMI>30 kg/m 2 ). All women underwent controlled ovarian hyper stimulation using long agonist protocol. Results : There were 88 (28.6%) in the normal weight group, 147 (47.7%) in the overweight and 73 (23.7%) in the obese group. All three groups were comparable with respect to age, duration of infertility, female and male causes of infertility. The three groups were similar with respect to day 2 LH/FSH levels, endometrial thickness and gonadotrophin requirements, oocyte quality, fertilization, cleavage rates, number of good quality embryos and clinical pregnancy rates. Conclusion :Increase in body mass index in women does not appear to have an adverse effect on IVF outcome. However, preconceptual counselling for obese women is a must as weight reduction helps in reducing pregnancy-related complications.

Keywords: Obesity, infertility, pregnancy outcome

How to cite this article:
Sathya A, Balasubramanyam S, Gupta S, Verma T. Effect of body mass index on in vitro fertilization outcomes in women. J Hum Reprod Sci 2010;3:135-8

How to cite this URL:
Sathya A, Balasubramanyam S, Gupta S, Verma T. Effect of body mass index on in vitro fertilization outcomes in women. J Hum Reprod Sci [serial online] 2010 [cited 2023 Feb 4];3:135-8. Available from:

   Introduction Top

Obesity is becoming a fast growing health problem across the world. In addition to diabetes and cardiovascular disease, it also leads to alterations in reproductive functions. Excess body fat leads to menstrual irregularities especially chronic oligo-anovulation and infertility. Obesity leads to hyperinsulinemia and consequent ovarian hyperandrogenism.

Obesity is also known to affect fertility. Several prospective studies have shown that obesity leads to anovulatory infertility. It is associated with higher miscarriage rates and higher prevalence of gestatational diabetes and pregnancy induced hypertension. [1],[2],[3]

In assisted reproduction, however, there are conflicting reports on the effect of obesity on oocyte quality, embryo development, lower number of mature oocytes, lower implantation and pregnancy rates. [4],[5],[6],[7],[8] Endometrium also seems to have a negative impact on reproductive outcome in studies based on oocyte donation model. The objective of our study is to assess the effect of women's BMI on the oocyte/embryo quality and the reproductive outcome of our non donor IVF/ICSI cycles.

   Materials and Methods Top

We conducted a retrospective analysis of the case records of 308 consecutive women who underwent non donor IVF cycles in our institute. They were classified into three groups based on their BMI i.e. normal weight (BMI<25 kg/m 2 ), overweight (25-30 kg/m 2 and obese (>30 kg/m 2 ). Potential confounding factors like the woman's age, origin of infertility and ovarian reserve were noted. Those women older than 40 years and with FSH more than 10mIU/ml were excluded from the analysis.

All women underwent controlled ovarian hyperstimulation using long agonist protocol. This involved initial down regulation with GnRH agonist from the mid luteal phase of the previous menstrual cycle and maintained till human chorionic gonadotrophin (hCG) administration. Recombinant FSH ± HMG was started from day 2/3 of next cycle and the dosage was decided based on the patient's age, antral follicle count, basal FSH/LH levels and response to previous ovarian stimulation. Their dosages were adjusted according to serum LH, estradiol levels and ovarian response. hCG was administered subcutaneously when at least 3 follicles were ≥ 18 mm in dimension. Oocyte retrieval was done 36 h later followed by embryo transfer on day 3.

Oocytes with expanded cumulus, radiant corona, distinct zona pellucida, clear cytoplasm, unfragmented first polar body and those without debris in the perivitelline space were considered as good. [9] After oocyte retrieval, fertilization was assessed the next day (day 1) and embryo cleavage 24 h later. Embryo morphology was studied on day 3 based onthe number of cells and fragmentation using the standard morphological criteria. A good day 3 embryo was defined as one with number of blastomeres greater than or equal to 8, fragmentation of < 20% and without multinucleated blastomeres. [10]

Pearson Chi-square tests were used to compare the binary variables between the various groups classified according to the BMI. Parametric tests were used for comparisons between the BMI groups and continuous variables. Analysis of variance (ANOVA) was then performed and post-hoc comparisons were made.

   Results Top

There was a total of 308 women. There were 88 (28.6%) in the normal weight group, 147 (47.7%) in the overweight and 73 (23.7%) in the obese group. 3 among the obese were morbidly obese. All three groups were comparable with respect of infertility [Table 1]. There was no significant difference in the day 2 gonadotrophin values (FSH, LH), endometrial thickness and gonadotrophin dose required for controlled ovarian stimulation [Table 2],[Figure 1]. The oocyte quality did not vary between the three groups [Figure 2]. The correlation studies between the body mass indices and the pregnancy rates, implantation rates and fertilization rates failed to show any significant association [Figure 3]. The number of missed abortions and ectopic pregnancies were comparable in the three groups [Table 3].
Table 1 :Baseline characteristics

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Table 2 :Day 2 LH/FSH levels, endometrial thickness and gonadotrophin requirements in the three groups

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Table 3 :Outcomes in the 3 BMI groups

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Figure 1 :Effect of BMI on LH, FSH, endometrial thickness and gonadotrophin dose

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Figure 2 :Effect of BMI on oocyte quality <25-48 good, 24 not good 25-30- 90 good, 26 not good, >30-16 good, 10 not good P = 0.12

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Figure 3 :Effect of BMI on pregnancy outcome <25 - 34 +, 38- , (total = 72), 25-30 - 52+, 64-, (total = 116), >30- 12+, 14-, (total = 26), P-0.95 (not significant)

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   Discussion Top

Anovulation has been the major cause of subfertility in obese women. [1] Reduced conception rates (5% per unit increase in BMI above 29 kg/m 2 ) have been described in normal cycling obese women as well. The effects of BMI in obese women undergoing controlled ovarian stimulation have been controversial. [1],[4],[5],[6]

Our study shows that BMI has no adverse effects over IVF outcome. The blood hormone parameters (day 2 gonadotrophin values, estradiol levels on hCG day) were similar in all three groups irrespective of weight. Obese women are expected to have high LH values owing to insulin resistance. However, we did not find the same in our study. Some studies have highlighted a state of gonadotrophin resistance in obese women leading to higher gonadotrophin requirement for COH. [4] Some studies have not found any adverse effects of obesity on ovarian response in IVF. Our study groups comprising overweight and obese women showed similar gonadotrophin requirements as that of normal weight women. Also, the number of days of controlled ovarian hyperstimulation was similar in all the three groups.

With regards to oocyte and embryo quality, some authors have reported poor quality among obese women. [8] Others have failed to find any association between the two.

Lacunae still exists as regards the role of endometrium, the embryo in implantation in obese women. Many studies indicate lower implantation and pregnancy rates, higher miscarriage rates and increase pregnancy- related complications for the mother and the fetus. [11] Nichols et al., report reduced conception rates in overweight women undergoing IVF. [2] Wang et al., also support the same. However, there have been conflicting conclusions from other studies. [3]

High miscarriage rates have been described in obese women conceiving naturally or after ART. A systematic review by Maheshwari et al., made similar observations in women with BMI greater than 25 kg/m 2 . [4] Metwally et al., in his meta analysis also observe similar trend in BMI greater than 25 kg/m 2 regardless of method of conception [8] In our study, we did not observe any difference between the three groups. This could be explained by the fact that the embryo quality was not affected by BMI.

Our study thus does not show any adverse effects of obesity on endometrial thickness, hormone levels, oocyte number and quality, implantation and pregnancy rates. The role of endometrium and factors related to embryo are still under study. Other confounding factors like the role of male obesity on semen quality have not been taken into account. We need to study the crosstalk between the endometrium and the embryo at molecular level and study obesity in male partner, the effect of obesity on semen quality and hence embryo quality.

We conclude that an increase in body mass index does not appear to have an adverse effect on IVF outcome. However, overweight and obese women must be counselled on weight reduction to reduce pregnancy-related complications.

   References Top

1.Norman RJ, Noakes M, Wu R. Improving reproductive performance in overweight/obese women with effective weight management. Hum Reprod Update 2004;10:267-80.  Back to cited text no. 1
2.Nichols JE, Crane MM, Higdon HL. Extremes of body mass index reduce in vitro fertilization rates. Fertil Steril 2003;79:645-7.  Back to cited text no. 2
3.Wang JX, Davies MJ, Norman RJ. Obesity increases the risk of spontaneous abortion during infertility treatment. Obes Res 2002;10:551-4.  Back to cited text no. 3
4.Maheshwari A, Stofberg L, Bhattacharya. Effect of overweight and obesity on assisted reproductive technology- a systematic review. Hum Reprod Update 2007;13:433-44.  Back to cited text no. 4
5.Fedorscak P, Dale PO, Storeng R. Impact of overweight and underweight on assisted reproduction treatment. Hum Reprod 2004;19:2523-8.  Back to cited text no. 5
6.Jose B, Yenira A, Marcos F. Female obesity impairs in vitro fertilization outcome without affecting embryo quality. Fertil Steril; 2009.  Back to cited text no. 6
7.Lewis CG, Warnes GM, Wang JX. Failure of body mass index or body weight to influence markedly the response to ovarian hyperstimulation in normal cycling women. Fertil Steril 1990;53:1097-9.  Back to cited text no. 7
8.Metwally M, Cutting R, Tipton A. Effect of increased body mass index on oocyte and embryo quality in IVF patients. Reprod Biomed Online 2007;15:532-8.  Back to cited text no. 8
9.Lin YC, Chang SY, Lan KC, Huang HW, Chang CY, Tsai MY, et al. Human oocyte maturity in vivo determines the outcome of blastocyst developmentin vitro.. J Assist Reprod Genet 2003;20:506-12.  Back to cited text no. 9
10.Gardner DK, Weissman A, Howles CM. Textbook of Assisted Reproductive Technologies. Informa UK Ltd: 2009. p. 244-5.   Back to cited text no. 10
11.Jungheim ES, Lanzendorf SE, Odem RR, Moley KH, Chang AS, Ratts VS. Morbid obesity is associated with lower clinical pregnancy rates after in vitro fertilization in women with polycystic ovarian syndrome. Fertil Steril 2009;92:256-61.  Back to cited text no. 11


  [Figure 1], [Figure 2], [Figure 3]

  [Table 1], [Table 2], [Table 3]

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