Journal of Human Reproductive Sciences

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 12  |  Issue : 2  |  Page : 122--129

Effect of spermatic nuclear quality on live birth rates in intracytoplasmic sperm injection


Maroua Hachemi1, Mustapha Bensaada2, Abdelkader Rouabah3, Abdelali Zoghmar5, Sebti Benbouhedja5, Leila Rouabah3, Mehdi Benchaib4 
1 Faculty of Nature and Life Sciences, Laboratory of Molecular and Cellular Biology, Frères Mentouri University Constantine I; Reproduction Sciences and Surgery Clinique, Ibn Rochd, Constantine, Algeria
2 Reproduction Sciences and Surgery Clinique, Ibn Rochd, Constantine; Faculty of Nature and Life Sciences, Abbès Laghrour University, Khenchela, Algeria
3 Faculty of Nature and Life Sciences, Laboratory of Molecular and Cellular Biology, Frères Mentouri University Constantine I, Constantine, Algeria
4 Hospices Civils de Lyon, HFME, Reproduction Biology Center, 59 Boulevard Pinel 69500 Bron, 5 Inserm U1208, 18 Avenue Doyen Lépine, 69675 Bron Cedex, Claude Bernard University, Lyon Est Faculty of Medicine, 8 Avenue Rockefeller, 69008, Lyon, France

Correspondence Address:
Maroua Hachemi
PhD Student, Faculty of Nature and Life Sciences, Laboratory of Molecular and Cellular Biology, Frères Mentouri University Constantine I
Algeria

Background: Our study defines the clinical role of sperm DNA damage in the assisted reproductive technology procedure. Aim: To investigate if the compaction of chromatin explored added to the analysis of the sperm DNA fragmentation allows obtaining a new indicator for sperm genome quality linked to live birth rate (LBR). Design: This was a prospective study, undergoing 101 cycles in the intracytoplasmic sperm injection (ICSI) program. Materials and Methods: The sperm DNA fragmentation index (DFI) has been measured with sperm chromatin dispersion examination. The sperm decondensation index (SDI) of chromatin has been measured with aniline blue procedure; with these indexes, a new parameter has been created: DFI × SDI. Statistical Analysis: Pearson's correlation coefficient, Student's t-test, and Chi-square test were used. The quantitative variables were described as mean ± standard deviation. Multivariate logistic regressions were performed with live birth as outcome. Results: The sperm concentration, motility, and normal morphology were lower when the DFI was high (P = 0.001). The fertilization rates and the number of obtained embryos were not statistically significant different according to the DFI groups. The SDI does not appear to be linked either with the spermatic parameters or with the ICSI parameters. A low DFI seems to be a beneficial factor to obtain a live birth in ICSI procedure (P = 0.064). In case of high DFI, a high SDI allows to obtain a higher LBR than a low SDI. Conclusion: The DFI is a good prognostic for a delivery rate in ICSI procedure, and the SDI could be added to DFI to create a new parameter of sperm nuclear quality. This new parameter seems to be linked to LBR.


How to cite this article:
Hachemi M, Bensaada M, Rouabah A, Zoghmar A, Benbouhedja S, Rouabah L, Benchaib M. Effect of spermatic nuclear quality on live birth rates in intracytoplasmic sperm injection.J Hum Reprod Sci 2019;12:122-129


How to cite this URL:
Hachemi M, Bensaada M, Rouabah A, Zoghmar A, Benbouhedja S, Rouabah L, Benchaib M. Effect of spermatic nuclear quality on live birth rates in intracytoplasmic sperm injection. J Hum Reprod Sci [serial online] 2019 [cited 2019 Aug 17 ];12:122-129
Available from: http://www.jhrsonline.org/article.asp?issn=0974-1208;year=2019;volume=12;issue=2;spage=122;epage=129;aulast=Hachemi;type=0