Journal of Human Reproductive Sciences

ORIGINAL ARTICLE
Year
: 2013  |  Volume : 6  |  Issue : 2  |  Page : 152--157

Efficacy of two sperm preparation techniques in reducing non-specific bacterial species from human semen


Prabath K Abeysundara1, DMAB Dissanayake1, Prasantha S Wijesinghe1, RRDP Perera2, AAN Nishad1 
1 Department of Obstetrics and Gynaecology, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka
2 Department of Medical Microbiology, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka

Correspondence Address:
Prabath K Abeysundara
No: 266/D, Polhena Road, Walpola, Ragama
Sri Lanka

Context: Artificial reproductive techniques using seminal preparations with bacteria may cause pelvic inflammatory disease and its sequalae. Aims: To assess efficacy of two sperm preparation techniques to clear bacteria and the effect of bacteriospermia on sperm recovery rates. Settings and Design: A descriptive cross-sectional study was carried out among males of subfertile couples. Subjects and Methods: Semen samples were randomly allocated into swim-up method (group S, n = 68) and density gradient method (group D, n = 50) for sperm preparation. Seminal fluid analysis and bacterial cultures were performed in each sample before and after sperm preparation. Statistical Analysis: McNemar«SQ»s chi-squared test and independent samples t-test in SPSS version 16.0 were used. Results: Organisms were found in 86 (72.88%) out of 118 samples, before sperm preparation; Streptococcus species (n = 40, 46.51% of which 14 were Group D Streptococcus species), Coagulase negative Staphylococcus species (n = 17, 19.76%), Staphylococcus aureus (n = 13, 15.11%), Coliform species (n = 11, 12.79% of which 09 were Escherichia coli) and Corynebacterium species (n = 5, 5.81%). There was a statistically significant reduction of culture positive samples in raw vs. processed samples; in group S, 49 (72.05%) vs. 16 (23.52%) and in group D, 37 (74%) vs. 18 (36%). In group S and D, mean (SD) recovery rates of culture positive vs. culture negative samples were 39.44% (SD-14.02) vs. 44.22% (SD-22.38), P = 0.39 and 52.50% (SD-37.16) vs. 49.58% (SD-40.32), P = 0.82 respectively. Conclusions: Both sperm preparation methods significantly reduced bacteria in semen, but total clearance was not achieved. Sperm recovery rate was not affected by bacteriospermia.


How to cite this article:
Abeysundara PK, Dissanayake D, Wijesinghe PS, Perera R, Nishad A. Efficacy of two sperm preparation techniques in reducing non-specific bacterial species from human semen.J Hum Reprod Sci 2013;6:152-157


How to cite this URL:
Abeysundara PK, Dissanayake D, Wijesinghe PS, Perera R, Nishad A. Efficacy of two sperm preparation techniques in reducing non-specific bacterial species from human semen. J Hum Reprod Sci [serial online] 2013 [cited 2020 Jun 3 ];6:152-157
Available from: http://www.jhrsonline.org/article.asp?issn=0974-1208;year=2013;volume=6;issue=2;spage=152;epage=157;aulast=Abeysundara;type=0