|Year : 2019 | Volume
| Issue : 3 | Page : 234-239
Comparative study of serum progesterone levels at the time of human chorionic gonadotropin trigger and ovum pickup in predicting outcome in fresh in vitro fertilization cycles
Srinivas Sangisapu, Sandeep Karunakaran
ART Centre, Institute of Naval Medicine, INHS Asvini, Mumbai, Maharashtra, India
|Date of Web Publication||16-Sep-2019|
Prof. Sandeep Karunakaran
ART Centre, Institute of Naval Medicine, INHS Asvini, Colaba, Mumbai - 400 005, Maharashtra
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Background: Studies have shown that premature rise of progesterone in controlled ovarian stimulation (COS) at the time of human chorionic gonadotropin (hCG) trigger is negatively associated with in vitro fertilization (IVF) outcome in fresh IVF cycles. Some authors have failed to demonstrate this. One large single centre retrospective cohort study has compared the pre and post hCG progesterone and observed that the ratio of the rise in progesterone could be a positive predictor. There is paucity of literature on this aspect. Aims and Objectives: To compare the serum progesterone at hCG trigger and ovum pick-up (OPU) with IVF outcome by estimating the respective paired hormone levels. Material and Methods: Serum progesterone levels at hCG trigger and OPU are compared retrospectively in 301 fresh IVF cycles with IVF outcome by long protocols with GnRH agonists for two years. Parametric and nonparametric testing of null hypothesis is performed. P value <0.05 is taken as significant. Results: There is no predictive association of IVF outcome with either progesterone levels. The ratio of rise in progesterone is strongly positively associated with IVF outcome (P < 0.001). However, after adjusting for confounders and modifiers the retrieved number of oocytes are positively associated with IVF outcome (P = 0.044). Conclusions: The ratio of rise in progesterone is significantly associated with number of oocytes retrieved which in turn is associated with successful IVF outcome in fresh cycles. Ratio of rise in progesterone seems to be therefore an indirect parameter for predicting successful IVF outcome in fresh cycles.
Keywords: Fresh in vitro fertilization cycles, in vitro fertilization outcome, serum progesterone at human chorionic gonadotropin trigger, serum progesterone at ovum pick up
|How to cite this article:|
Sangisapu S, Karunakaran S. Comparative study of serum progesterone levels at the time of human chorionic gonadotropin trigger and ovum pickup in predicting outcome in fresh in vitro fertilization cycles. J Hum Reprod Sci 2019;12:234-9
|How to cite this URL:|
Sangisapu S, Karunakaran S. Comparative study of serum progesterone levels at the time of human chorionic gonadotropin trigger and ovum pickup in predicting outcome in fresh in vitro fertilization cycles. J Hum Reprod Sci [serial online] 2019 [cited 2020 Jan 25];12:234-9. Available from: http://www.jhrsonline.org/text.asp?2019/12/3/234/266892
| Introduction|| |
In clinical practice, the ovulation trigger of mid-cycle luteinizing hormone (LH) surge is usually achieved by human chorionic gonadotropin (hCG) due to its molecular similarity, binding to LH receptors, and longer half-life. hCG trigger induces luteinization of mature follicle and formation of corpus luteum. The primary role of progesterone produced by corpus luteum is the preparation of estrogen primed endometrium for implantation of an embryo and its maintenance. There of till the initial weeks of pregnancy. Progesterone also plays a role in the expression of genes needed for implantation at the level of the endometrium., It is seen in controlled ovarian stimulation (COS) protocols that there is premature release of progesterone in the late follicular phase due to simultaneous stimulation of large number of follicles resulting in asynchronous endometrium and implantation failure in fresh cycles., Several studies have observed that this premature progesterone rise in gonadotropin-releasing hormone (GnRH) downregulated cycles on the day of hCG trigger is negatively associated with in vitro fertilization (IVF) outcome.,,, Other investigators failed to support this view. Few other studies evaluated the paired samples of serum progesterone levels at the time of hCG trigger and post-hCG administration to determine which one correlated better with IVF outcome. So far, only three studies are available.,, There is a paucity of literature on this front and hence the present study.
Aim of the study
The aim is to compare serum progesterone levels at hCG trigger and ovum pick up (OPU) in predicting successful IVF outcome in fresh IVF cycles.
Objectives of the study
To estimate and evaluate the correlation of serum progesterone levels at hCG trigger and OPU with successful IVF outcome.
To evaluate which among the above two is a better predictor for a successful IVF outcome.
| Subjects and Methods|| |
A single-center retrospective cohort study is conducted on 306 fresh IVF cycles of normozoospermic semen samples and COS by long protocol with GnRH agonists followed by hCG trigger from 2016 to 2018.
All couples underwent complete evaluation including detailed history, physical examination, blood counts, urinalysis, hormone profile, blood sugar, blood group, coagulation profile, viral markers, serological tests for syphilis, hormone profile, Mantoux test, erythrocyte sedimentation rate, chest X-ray, hysterosalpingogram, ultrasonography of pelvis, ovulation studies, evaluation of ovarian reserve, diagnostic hystero-laparoscopy, and semen analysis (at least twice) as per the WHO manual 2010. All female partners received preconceptional folic acid supplementation 5 mg twice daily and tablet aspirin 75 mg once daily.
Cycles with the retrieval of at least three mature oocytes and fresh semen preparations recovering morphologically normal sperms of a concentration ≥10 million/ml are included in the study.
Cycles with poor/nonresponse to COS and fresh semen preparations recovering morphologically normal sperms of a concentration <10 million/ml are excluded from the study.
Additional inclusion and exclusion criteria with reasons are listed in [Box 1].
COS is done by individualizing the starting dose of gonadotrophins based on age, basal follicle-stimulating hormone (FSH) levels, body mass index, and response during earlier stimulated cycles. Depending on ovarian response by ultrasound assessment, dose adjustments of gonadotrophins are carried out after at least 5 days of stimulation. When the follicular size is at 18–20 mm, 250 mcg of recombinant hCG and 5000 IU urinary hCG are administered for final maturation of the oocyte. OPU is carried out after 36 h of hCG trigger.
Insemination is performed using droplet method with oil overlay within 1 h of OPU/removing sperms from the final swim up, and embryos are cultured in sequential culture media (vitrolife).
The assessment of fertilization is made on day 1, i.e., 18–20 h postinsemination at 2-pronuclei stage. Denudation is carried out on day 1 at the time of assessment of fertilization. Day 2 embryos with 2–4 cells and day 3 embryos with 6–8 cells, and <20% fragmentation is taken as good-quality embryos (Grades 1 and 2), embryo transfer (ET) is performed at the cleavage stage (day 3) of the embryo development. One to three in number of embryos is transferred depending on the yield and quality of embryos.
Post-OPU and post-ET luteal phase support are given by micronized progesterone injections 100 mg once daily, and tablet dydrogesterone 10 mg twice a day from the day of OPU until the 12th week of gestation, if pregnancy is tested positive.
Positive or negative pregnancy as tested by serum beta hCG (≥100 mIU/ml) on day 17 of ET is taken as the primary outcome variable. Number of mature oocytes retrieved is taken as an intermediate outcome/secondary outcome.
Paired serum samples are collected from female partners at hCG trigger and at OPU. The samples underwent radioimmunoassay (RIA) using Beckman Coulter RIA kit for measuring serum progesterone level. The analytical sensitivity was 0.11 nmol/L intra- and inter-assay precision, expressed as coefficients of variation were 6.7% and 17.3%, respectively. The tests are done after taking ethical committee approval along with written and informed consent of patients. Staff at assisted reproductive technology center are blinded to progesterone levels. Staff at endocrine laboratory measuring the progesterone values are blinded to IVF outcome. Serum progesterone levels and IVF outcome are analyzed using retrospectively by the third group of investigators.
Statistical analysis is performed using the IBM SPSS statistics 20 (SPSS Inc., Armonk, New York, USA). Proportions are compared using the Chi-square test and means using independent samples t-test and analysis of variance. Binary logistics regression performed for adjusting the effect of confounders, modifiers, and moderators. We have considered parametric tests because of the large sample size, different spread/dispersion of compared groups, and to give better statistical power to study if there is a true significance effect that exits. We have also performed simultaneously non parametric tests to test the Null Hypothesis since many of the variables were following non normal distribution pattern. Value of P < 0.05 is considered as statistically significant.
| Results|| |
Three-hundred and one fresh IVF cycles of 306 [Flow Chart] are analyzed with variable parameters-age, duration of infertility, serum progesterone at hCG trigger and OPU, the ratio of rise in serum progesterone, number of retrieved mature fertilizable oocytes, and IVF outcome.
Baseline characteristics – [Table 1] depicts the baseline characteristics of the independent variables of our study.
Overall IVF outcome – 154 (51%) of 301 are tested positive for pregnancy, and 147 (49%) are tested negative.
Summary of bivariate analysis by parametric tests [Table 2]
[Table 2] shows the relationship of each independent variable with IVF outcome, P value and statistical significance. In the present study, it is seen that serum progesterone levels at hCG trigger, and OPU independently have no significant association with IVF outcome. However, the ratio of rise in progesterone is strongly associated with positive IVF outcome. It is also seen that the serum progesterone levels at hCG trigger have no significant association with a number of mature oocytes retrieved. However, serum progesterone at OPU, the ratio of rise in progesterone is positively associated with the number of mature oocytes retrieved which in turn have a significant positive association with pregnancy.
Summary of bivariate analysis by non parametric tests [Table 2]
We have also performed nonparametric tests taking into consideration that the median of the many of the groups actually represented the measure of central tendency [Table 2]. These tests included independent median test, Mann–Whitney U-test, Kolmogorov–Smirnov test, Kruskal–Wallis test, and Jonckheere–Terpstra test. All these tests show significant (<0.05) positive association of number of oocytes retrieved with positive IVF outcome. However, there is no direct association with either stand-alone progesterone values at hCG trigger, at OPU or the ratio of rise of these paired samples with IVF outcome. However, related samples Wilcoxon signed-rank test additionally show significant association of stand-alone progesterone value at hCG, at OPU, and their ratio of rise to the number of oocytes retrieved which, in turn, is associated with positive IVF outcome.
Binary logistic regression
The primary outcome is further analyzed using binary logistic regression with age groups, duration of infertility, the ratio of rise in serum progesterone, and number of oocytes retrieved [Table 3]. The number of oocytes after adjusting for age groups, duration of infertility, and the ratio of rise in progesterone is significantly positively associated with the pregnancy (P = 0.044).
| Discussion|| |
Bivariate analysis [Table 2] of two primary independent variables of our study progesterone levels at the time of hCG trigger and at OPU are not found to be associated with IVF outcome. Premature rise of progesterone before hCG trigger is shown to have a negative association with the pregnancy in fresh IVF cycles.,,, The incidence of rise in serum progesterone on or before the day of hCG trigger is 35% (5%–35%) in GnRH agonist cycles and 38% (9%–38%) in GnRH antagonist cycles.,,,, Serum progesterone rise on the day of hCG has been a matter of debate with studies showing contrary evidence. Some studies have shown no correlation with the IVF outcome. Venetis CA et al are of the opinion that this apparent lack of association of premature rise of serum progesterone at the time of hCG trigger with IVF outcome could be due to failure to adjust for confounders and modifiers by multivariate regression. Endometrial receptivity is related the hormonal milieu during ET. High concentrations of estradiol during COS result in premature elevation of progesterone, asynchronous endometrium, and implantation failure. This negative effect of progesterone elevation is limited to the endometrium. There is no effect on oocyte maturation or fertilization as corroborated by donor-recipient IVF cycles and in frozen embryo cycles. Vitrification of embryos can be a useful alternative approach when progesterone levels exceed a suggested threshold (1.5 ng/ml).
While stand-alone serum progesterone levels do not show significant association with successful IVF outcome, the ratio of rise in serum progesterone in the paired samples is significantly strongly associated with positive IVF outcome [Table 2]. However, it is also to be noted that analysis by Mann–Whitney U-test does not show significant association [P = 0.912; [Table 2]. Nevertheless, this is an important observation of our study. The aim of the study is to make a comparative analysis of serum progesterone at hCG trigger and at OPU to find out how they are associated with IVF outcome and therefore serve as predictors of successful IVF outcome in fresh IVF cycles. The ratio of rise in progesterone has emerged as a predictor instead. However, the significance is lost after adjusting for number of oocytes, age, and duration of infertility [Table 3]. Moreover, analysis by non parametric tests does not show the significance. Zhu et al. in their single-center retrospective analysis of the first fresh IVF cycles of a large cohort of women aged 24–43 years among other findings, conclude that the ratio of change in post-hCG progesterone could be used as another indirect parameter to estimate the pregnancy rate and live birth rate. The primary outcome studied was the effect of this change in post-hCG progesterone levels with number and quality of oocytes and quality of embryos. The implantation rates and pregnancy rates were also studied. The investigators further stratified the ratio of rise to seven subgroups and found that the subgroup of patients with higher ratio of rise in progesterone has higher chances of retrieval of good number of oocytes therefore indirectly live birth rates. Besides, the above-quoted study there are only two more studies of small group of patients., Our study seems to be the only study to estimate the post-hCG serum progesterone on the day of OPU.
Elevated serum progesterone levels at hCG trigger show no significant (P = 0.051) positive association with number of oocytes recovered by parametric tests although non parametric test shows a significant association (P=<0.001) [Table 2]. However, progesterone levels at OPU and ratio of rise are found to be significantly associated with number of oocytes retrieved by both parametric and non parametric tests (P = 0.038 and < 0.001) [Table 2]. Number of oocytes, in turn, is positively associated with positive IVF outcome both by bivariate analysis (P = 0.022) [Table 2] and by binary logistic regression (P = 0.044) [Table 3].
Zhu et al. observed that post-hCG progesterone levels were positively associated with the number of oocytes retrieved, but did not affect oocyte or embryo quality. The effect of premature progesterone rise on IVF outcome may be entirely different depending on strong or weak response to COS. Low pregnancy rates were reported when the ovarian response was weak. We need to consider ovarian reserve, oocyte, and embryo quality which could alter the IVF outcome vis-a-vis serum progesterone rise at hCG., Several studies have also shown that strong premature progesterone rise at hCG trigger is linked to the quantum of ovarian response to stimulation.,,,,,
Ovarian response may be having a moderating influence on the premature rise of progesterone and the pregnancy rates. Pooled data published by Griesinger et al. from six clinical trials suggested that premature rise of progesterone at hCG does not compromise pregnancy rates in high responders. However, Bosch et al. and Xu et al. showed deleterious effects of progesterone elevation at hCG to ongoing pregnancy rates regardless of the magnitude of ovarian response. Large multivariate analysis of 3296 fresh IVF cycles after adjusting for several confounders have shown that there is no evidence to support a moderating effect of ovarian response on the association of progesterone elevation at hCG trigger with live birth rates. However, the authors were of the opinion that there could still be a Type II error.
| Conclusion|| |
Stand-alone serum progesterone levels either at hCG trigger or at OPU are not associated with IVF outcome. Serum progesterone at OPU and the ratio of rise of progesterone are significantly associated with number of oocytes retrieved which in turn is associated with successful IVF outcome in fresh cycles and therefore seem to be indirect parameters for predicting successful IVF outcome. This is in agreement with another large retrospective cohort study. Apart from our study and the large cohort mentioned, there are only two small group of studies comparing the paired samples of serum progesterone pre- and post-hCG trigger. Ours is unique study in the sense, we are the only investigators who analyzed post-hCG values at the time of OPU.
Limitations of the study
- Single-center study
- Retrospective study
- Secondary outcomes such as implantation rate, miscarriage rate, and live birth are not included in the study
- Limited number of independent variables are included in the binary logistic regression to find out adjusted association/relationship
- Multivariate regression analysis is not carried out.
Therefore, we recommend multicentric prospective studies including all possible confounders, modifiers, and moderators such as COS by GnRH protocols (agonist and antagonist), basal FSH, E2 and progesterone levels, total dose of gonadotrophins administered, number of mature oocytes retrieved, number and quality and number of embryos transferred.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Homburg R. Ovulation Induction and Controlled Ovarian Stimulation: A Practical Guide. 2nd
ed. Cham: Springer; 2014.
Murphy BD. Models of luteinization. Biol Reprod 2000;63:2-11.
Van Vaerenbergh I, Fatemi HM, Blockeel C, Van Lommel L, In't Veld P, Schuit F, et al.
Progesterone rise on HCG day in GnRH antagonist/rFSH stimulated cycles affects endometrial gene expression. Reprod Biomed Online 2011;22:263-71.
Labarta E, Martínez-Conejero JA, Alamá P, Horcajadas JA, Pellicer A, Simón C, et al.
Endometrial receptivity is affected in women with high circulating progesterone levels at the end of the follicular phase: A functional genomics analysis. Hum Reprod 2011;26:1813-25.
Allahbadia GN. To transfer or not to transfer: That is the question. IVF Lite 2014;1:6974. [Full text]
Bosch E, Valencia I, Escudero E, Crespo J, Simón C, Remohí J, et al.
Premature luteinization during gonadotropin-releasing hormone antagonist cycles and its relationship with in vitro
fertilization outcome. Fertil Steril 2003;80:1444-9.
Kiliçdag EB, Haydardedeoglu B, Cok T, Hacivelioglu SO, Bagis T. Premature progesterone elevation impairs implantation and live birth rates in GnRH-agonist IVF/ICSI cycles. Arch Gynecol Obstet 2010;281:747-52.
Venetis CA, Kolibianakis EM, Bosdou JK, Tarlatzis BC. Progesterone elevation and probability of pregnancy after IVF: A systematic review and meta-analysis of over 60 000 cycles. Hum Reprod Update 2013;19:433-57.
Zhu H, Liu L, Yang L, Xue Y, Tong X, Jiang L, et al.
The effect of progesterone level prior to oocyte retrieval on the numbers of oocytes retrieved and embryo quality in IVF treatment cycles: An analysis of 2,978 cycles. J Assist Reprod Genet 2014;31:1183-7.
Burns WN, Witz CA, Klein NA, Silverberg KM, Schenken RS. Serum progesterone concentrations on the day after human chorionic gonadotropin administration and progesterone/oocyte ratios predict in vitro
fertilization/embryo transfer outcome. J Assist Reprod Genet 1994;11:17-23.
Check JH, Amui J, Choe JK, Brasile D. Relationship of serum progesterone (P) level the day after human chorionic gonadotropin (hCG) injection on outcome following in vitro
fertilization-embryo transfer (IVF-ET). Clin Exp Obstet Gynecol 2009;36:214-5.
Ubaldi F, Camus M, Smitz J, Bennink HC, Van Steirteghem A, Devroey P. Premature luteinization in in vitro
fertilization cycles using gonadotropin-releasing hormone agonist (GnRH-a) and recombinant follicle-stimulating hormone (FSH) and GnRH-a and urinary FSH. Fertil Steril 1996;66:275-80.
Silverberg KM, Burns WN, Olive DL, Riehl RM, Schenken RS. Serum progesterone levels predict success of in vitro
fertilization/embryo transfer in patients stimulated with leuprolide acetate and human menopausal gonadotropins. J Clin Endocrinol Metab 1991;73:797-803.
Edelstein MC, Seltman HJ, Cox BJ, Robinson SM, Shaw RA, Muasher SJ. Progesterone levels on the day of human chorionic gonadotropin administration in cycles with gonadotropin-releasing hormone agonist suppression are not predictive of pregnancy outcome. Fertil Steril 1990;54:853-7.
Bosch E, Labarta E, Crespo J, Simón C, Remohí J, Jenkins J, et al.
Circulating progesterone levels and ongoing pregnancy rates in controlled ovarian stimulation cycles for in vitro
fertilization: Analysis of over 4000 cycles. Hum Reprod 2010;25:2092-100.
Ochsenkühn R, Arzberger A, von Schönfeldt V, Gallwas J, Rogenhofer N, Crispin A, et al.
Subtle progesterone rise on the day of human chorionic gonadotropin administration is associated with lower live birth rates in women undergoing assisted reproductive technology: A retrospective study with 2,555 fresh embryo transfers. Fertil Steril 2012;98:347-54.
Venetis CA, Kolibianakis EM, Bosdou JK, Lainas GT, Sfontouris IA, Tarlatzis BC, et al.
Estimating the net effect of progesterone elevation on the day of hCG on live birth rates after IVF: A cohort analysis of 3296 IVF cycles. Hum Reprod 2015;30:684-91.
Fanchin R, Righini C, Olivennes F, Ferreira AL, de Ziegler D, Frydman R. Consequences of premature progesterone elevation on the outcome of in vitro
fertilization: Insights into a controversy. Fertil Steril 1997;68:799-805.
Younis JS. ElevatedPlevel on the day of hCG administration is related to FSH dose: Is it the whole truth? Hum Reprod 2011;26:498-9.
de Ziegler D, Bijaoui G, Chapron C. Pre-hCG elevation of plasma progesterone: Good, bad or otherwise. Hum Reprod Update 2008;14:393.
Lindheim SR, Cohen MA, Chang PL, Sauer MV. Serum progesterone before and after human chorionic gonadotropin injection depends on the estradiol response to ovarian hyperstimulation during in vitro
fertilization-embryo transfer cycles. J Assist Reprod Genet 1999;16:242-6.
Al-Azemi M, Kyrou D, Kolibianakis EM, Humaidan P, Van Vaerenbergh I, Devroey P, et al.
Elevated progesterone during ovarian stimulation for IVF. Reprod Biomed Online 2012;24:381-8.
Sunkara SK, Rittenberg V, Raine-Fenning N, Bhattacharya S, Zamora J, Coomarasamy A. Association between the number of eggs and live birth in IVF treatment: An analysis of 400 135 treatment cycles. Hum Reprod 2011;26:1768-74.
Griesinger G, Mannaerts B, Andersen CY, Witjes H, Kolibianakis EM, Gordon K. Progesterone elevation does not compromise pregnancy rates in high responders: A pooled analysis of in vitro
fertilization patients treated with recombinant follicle-stimulating hormone/gonadotropin-releasing hormone antagonist in six trials. Fertil Steril 2013;100:1622-80.
Xu B, Li Z, Zhang H, Jin L, Li Y, Ai J, et al.
Serum progesterone level effects on the outcome of in vitro
fertilization in patients with different ovarian response: An analysis of more than 10,000 cycles. Fertil Steril 2012;97:1321-70.
[Table 1], [Table 2], [Table 3]