|Year : 2016 | Volume
| Issue : 1 | Page : 53-55
Successful pregnancy following single blastocyst transfer in a renal transplant recipient
V Arun Muthuvel1, Manipriya Ravindran1, Aravind Chander2, Chandralekha Veluswamy1
1 Department of Reproductive Medicine, Iswarya Women's Hospital and Fertility Centre, Chennai, Tamil Nadu, India
2 Department of Reproductive Medicine, Iswarya Women's Hospital and Fertility Centre, Coimbatore, Tamil Nadu, India
|Date of Submission||20-Sep-2015|
|Date of Decision||28-Dec-2015|
|Date of Acceptance||28-Dec-2015|
|Date of Web Publication||14-Mar-2016|
Dr. V Arun Muthuvel
Department of Reproductive Medicine, 1st Main Road, Kasturba Nagar, Adyar, Chennai - 600 020, Tamil Nadu
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Numerous spontaneous pregnancies have been reported in renal transplant recipients; however, only a few pregnancies after the use of assisted reproductive techniques. The authors report a case of renal transplant recipient with secondary infertility who delivered a healthy baby without any complications. The report highlights the importance of minimal stimulation protocol during ovarian stimulation, single embryo transfer, and the need for multispecialty care for these patients. To the best of the authors' knowledge, the present report is the first such case from India and also the second in the world to report a blastocyst transfer among renal transplant recipients.
Keywords: In vitro fertilization, infertility, renal transplant, single embryo blastocyst transfer
|How to cite this article:|
Muthuvel V A, Ravindran M, Chander A, Veluswamy C. Successful pregnancy following single blastocyst transfer in a renal transplant recipient. J Hum Reprod Sci 2016;9:53-5
|How to cite this URL:|
Muthuvel V A, Ravindran M, Chander A, Veluswamy C. Successful pregnancy following single blastocyst transfer in a renal transplant recipient. J Hum Reprod Sci [serial online] 2016 [cited 2020 Mar 29];9:53-5. Available from: http://www.jhrsonline.org/text.asp?2016/9/1/53/178633
| Introduction|| |
Clinical management of infertility among organ transplant recipients is a challenge to the treating physician with hitherto limited information in literature. In particular, artificial reproductive techniques (ART) have been seldom attempted among these patients due to the possibility of life-threatening complications. The tremendous progress in the fields of ART and transplantation medicine offers the opportunity to safely explore various options for pregnancy. The authors present a case of a young postrenal transplant female with secondary infertility who successfully delivered a healthy baby following a single blastocyst transfer.
| Case Report|| |
A 30-year-old woman, married for 8 years was referred with a desire to conceive. At the age of 23, she conceived spontaneously but suffered a miscarriage. Following this pregnancy, she failed to conceive naturally. She underwent renal transplant for immunoglobulin A nephropathy at the age of 26. At the time of presentation to the authors, she was on tacrolimus, azathioprine, and prednisolone. Her serum blood urea nitrogen and creatinine were within normal limits (14 mg/dl and 0.8 mg/dl, respectively). Other clinical and biochemical parameters were found to be normal. Ultrasound with Doppler revealed normal transplant kidney with mild polycystic ovary disease. The patient's basal hormone profile worked on day three of menstrual cycle as follows: Luteinizing hormone (LH) - 8.28 mlU/mL, follicle-stimulating hormone (FSH) - 4.04 mlU/mL, prolactin - 19.03 ng/mL, and anti-mullerian hormone - 4.1 ng/mL. Antral follicular count was more than 24 follicles indicative of polycystic ovarian morphology. Semen analysis of the patient's husband was normal. Hence, intra-uterine insemination was tried for four cycles. All the cycles were ovulatory with appropriate endometrial lining. However, she failed to conceive.
The option of in vitro fertilization (IVF) was opted by the couple after being fully counseled of the potential risks associated with ART and pregnancy. Following a discussion with the nephrologists and high-risk pregnancy specialist, IVF cycle was planned and initiated without precycle suppression with oral contraceptive pills. A mild stimulation protocol with recombinant FSH 150 IU/day (injection Gonal F, Merck KGaA) was started on day 3 and continued for 5 days. Follicular monitoring was started on day 6. Dose adjustment was done on day 8. Recombinant FSH dose was reduced to 75 IU/day and continued till day 11. Gonadotropin-releasing hormone (GnRH) antagonist, injection Cetrorelix acetate 250 mcg (injection Cetrotide, Merck KGaA) was administered subcutaneously on menstrual cycle day 7 and continued until ovulation trigger. She developed 12 follicles, each more than 16 mm in size. Ovulation was induced with injection Triptorelin 0.2 mg (injection Decapeptyl 0.2 mg, Ferring pharmaceuticals), when more than 3 follicles measured >17 mm. After 35 h, oocyte retrieval was done under ultrasound guidance with intravenous sedation. Injection human chorionic gonadotropin (HCG) 1500U was administered immediately after oocyte retrieval to rescue the luteal phase. Then, 10 mature oocytes were retrieved and all the 10 oocytes were subjected to ICSI. On day 3, eight excellent quality embryos developed and extended culture was done till day 5. Endometrial thickness was 8 mm and a single expanded blastocyst was transferred with Labotect catheter. Seven other good quality blastocysts were cryopreserved.
Luteal phase support was started 24 h after oocyte retrieval and continued until 8 weeks with 90 mg progesterone gel (Crinone 8% gel, Merck KGaA) once a day. The patient did not develop any signs or symptoms of ovarian hyperstimulation syndrome (OHSS). Serum beta HCG levels were estimated to be 800 IU on day 14 of embryo transfer. Transvaginal ultrasound was performed 2 weeks after embryo transfer and single viable fetus corresponding to 6 weeks was observed. Throughout the treatment cycle and during pregnancy, the patient's anti-rejection drugs (prednisolone, tacrolimus, and azathioprine) were continued at maintenance doses.
The patient was monitored vigilantly and the entire course of pregnancy was uneventful with normal blood pressure and renal function. There was no proteinuria observed during weekly urine monitoring and the patient did not develop any signs of preeclampsia. In addition, growth of the fetus was monitored at regular intervals. Cesarean section was performed electively at 37 weeks and the patient delivered a healthy male baby weighing 3.2 kg. There was no evidence of graft rejection thereafter.
| Discussion|| |
In 1995, the first successful IVF in a renal transplant recipient was performed by Lockwood et al. The first successful blastocyst transfer in a renal transplant recipient was reported in 2008 by Fichez et al. To the best of the authors' knowledge, there have been only 7 cases reported till the year 2015, on successful pregnancies after IVF in renal transplant recipients. Renal insufficiency disrupts the normal gonadal function due to dysfunction of hypothalamo-pituitary-gonadal axis resulting in high FSH, LH, and prolactin levels. This hormonal pattern results in infertility. After successful transplantation, subsequent spontaneous pregnancies have been reported in 12% of the women in reproductive age. Many studies have claimed that the fertility is restored in 1 to 3 years following transplantation. The risk of graft rejection is high during the 1st year and therefore it is not advised to plan IVF within that period.
This was the only case to have safely delivered a 37 weeks term baby without any maternal and neonatal complications. Maternal complications during pregnancy are pregnancy-induced hypertension, preeclampsia, deep vein thrombosis, infections, and graft rejection while the fetal complications include preterm birth, very low birth weight, intrauterine growth restriction, or small for gestational age babies. OHSS is one the complications that can arise during ovulation induction. This can be attributed to the altered metabolism of the recombinant FSH which in turn is due to the impaired renal function. Further, enlarged ovaries can rarely obstruct the transplanted kidney during induction resulting in deterioration of renal function.
The present case report did not develop OHSS despite having polycystic ovaries because of the mild stimulation protocol with vigilant monitoring. Step down protocol with reduction in gonadotropin dosage was used. The risk of OHSS was reduced to a significant extent by triggering final oocyte maturation with GnRH agonist trigger and using small dose of HCG after oocyte retrieval. Single embryo transfer is recommended while the remaining embryos could be cryopreserved to avoid complications that arise from multiple pregnancies.
It is also recommended that transplant recipients satisfy certain criteria before contemplating pregnancy to avoid complications [Figure 1]. Although pregnancy causes an increase in glomerular filtration rate, hyperfilteration is compensated by the normal intraglomerular pressure. Therefore, no glomerular damage occurs if allograft function is normal prior to pregnancy. According to Alston et al., the graft rejection rate among posttransplant pregnancies is 6%, which is similar to that of a nonpregnant population. With more such successful cases, the number of transplant recipients seeking assisted reproduction is likely to increase.
|Figure 1: Criteria to consider pregnancy among renal transplant recipients|
Click here to view
| Conclusion|| |
With increasing cases of renal transplantation, women approaching for infertility treatment are expected to increase and more complicated cases have to be dealt with. It is safe to plan pregnancy through ART for renal transplant recipients under vigilant multi-specialty care. It is prudent to initiate mild stimulation protocol and trigger final oocyte maturation with a GnRH agonist to avoid complications such as OHSS. Single embryo transfer is recommended to avoid the complications of multiple pregnancies.
We extend our sincere gratitude to Dr. Veluswamy and his team of doctors for their contribution in managing the case.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
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