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ORIGINAL ARTICLE  
Year : 2015  |  Volume : 8  |  Issue : 3  |  Page : 159-164
 

Microdeletion of Y chromosome as a cause of recurrent pregnancy loss


1 Department of Obstetrics and Gynaecology, Teerthanker Mahaveer Medical College and Research Centre, TMU, Moradabad, India
2 Department of Radiology, Teerthanker Mahaveer Medical College and Research Centre, TMU, Moradabad, India
3 Department of Obstetrics and Gynaecology, Institute of Medical Sciences, BHU, Varanasi, Uttar Pradesh, India
4 Department of Molecular and Human Genetics, BHU, Varanasi, Uttar Pradesh, India

Date of Submission25-Nov-2014
Date of Decision04-May-2015
Date of Acceptance13-Aug-2015
Date of Web Publication11-Sep-2015

Correspondence Address:
Arjit Agarwal
A 46, Gandhi Nagar, Moradabad 244 001, Uttar Pradesh
India
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Source of Support: Nil., Conflict of Interest: There are no conflicts of interest.


DOI: 10.4103/0974-1208.165145

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   Abstract 

CONTEXT: In majority of couples experiencing recurrent pregnancy loss (RPL), etiology is still unknown. Two genetic factors have been suggested to underlie miscarriage in a subset of patients, namely skewed X chromosome inactivation in females and Y chromosome microdeletions in their partners. In males, microdeletions of the Y chromosome are known to cause spermatogenetic failure and male infertility. AIMS: The aim of the study was to find out the role of Y chromosome microdeletion in male partners of couples experiencing RPL. SETTINGS AND DESIGN: University hospital and genetic laboratory. Prospective case–control study. SUBJECTS AND METHODS: 59 couples with a history of RPL and 20 fertile controls (FC) with no miscarriage were included in the study. The study subjects were divided into male partners of RPL couples with abnormal semen parameters (AS) (n = 8), and couples with normal semen parameters (NS) (n = 51). Fertile controls with normal semen parameters were (FC) (n = 20). Y chromosome microdeletion was performed on 40 male partners of RPL and 20 FC. STATISTICAL ANALYSIS USED: Chi-square test. P <0.05 were considered statistically significant. RESULTS: 13 of the 40 RPL cases showed deletion in three azoospermia factor loci on the long arm of Y chromosome. The P value was significant with Y chromosome microdeletion in RPL cases as compared to 20 FC where no Y chromosome microdeletion was present. CONCLUSIONS: Y chromosome microdeletion may be an important hidden cause of recurrent pregnancy miscarriage and can be offered to couples with the undiagnosed cause of miscarriage.


Keywords: Recurrent pregnancy miscarriage, semen analysis, Y chromosome microdeletion


How to cite this article:
Agarwal S, Agarwal A, Khanna A, Singh K. Microdeletion of Y chromosome as a cause of recurrent pregnancy loss. J Hum Reprod Sci 2015;8:159-64

How to cite this URL:
Agarwal S, Agarwal A, Khanna A, Singh K. Microdeletion of Y chromosome as a cause of recurrent pregnancy loss. J Hum Reprod Sci [serial online] 2015 [cited 2020 May 28];8:159-64. Available from: http://www.jhrsonline.org/text.asp?2015/8/3/159/165145





   Introduction Top


Recurrent pregnancy loss (RPL), defined as the loss of three or more pregnancies, is experienced by approximately 1–2% of couples trying to conceive.[1] Several factors, such as genetic, anatomic, thrombophilic, endocrine, or immune, are known to contribute to RPL. Even after routine investigations, up to 50% of RPL cases remain unexplained.[2],[3]

Skewed X chromosome inactivation in females [4],[5] and Y chromosome microdeletions in their partners [6] have been suggested as a cause of miscarriage. Y chromosome microdeletion is known to cause spermatogenetic failure and male infertility.[7],[8] Y chromosome microdeletions occur in at least three regions, called azoospermia factor (AZFa), AZFb, and AZFc. 10 recurrent deletions in the three AZF regions have been described in detail [9] which may lead to varying degrees of spermatogenic failure. AZF deletions have a negative impact on the sperm quality and abnormal spermatozoa that may be associated with RPL.[10] Based on previous studies, the complete deletion of the AZFb and AZFc may have a direct effect on early prophase and decrease the rate of normal pairing in pachytene stage of spermatocytes. AZFc region deletion shows variation in clinical phenotype and testicular histology. Surprisingly, little is known about the function of the individual genes and transcription units in these regions.[8],[11] Y chromosome microdeletions are detected in approximately 7% of men with oligozoospermia.[9] Dewan et al.[6] conducted a study to determine if the Y chromosome microdeletions are associated with RPL and found a significant portion of male partners of women with RPL had Y-chromosomal microdeletions thereby suggesting it as an important cause of maintaining gestation.

Usually, semen analysis is thefirst diagnostic step in the evaluation of male factor as a cause of recurrent pregnancy miscarriage. Sperms play a critical role in early embryogenesis, and thus its role extends beyond fertilization in postimplantation events and embryogenesis.[12],[13],[14] It is possible that changes in molecular structure of the sperm may contribute to abortions, hence sperm DNA integrity is one of the important characteristic features.[15] With the above-mentioned background in mind, this study was conducted to find out male factor as a cause of RPL (semen analysis, Y chromosome microdeletion) and to study the correlation of semen analysis with Y chromosome microdeletion, in cases of RPL versus fertile male as controls.


   Subjects and Methods Top


The study included 59 males, whose female partner was diagnosed with the history of three or more pregnancy miscarriages prior to 20 weeks of gestation. The subjects were randomly selected from the outpatient department over the span of 2 years. Detailed medical, lifestyle, and family history of both partners were recorded in a predesigned proforma. The study was approved by the Institute Ethical Committee of Institute of Medical Sciences, and informed consent was obtained from each couple. The female partners were evaluated for complete gynecological, clinical, and laboratory investigations to rule out endocrine disorders, anatomical defects, chromosomal, immunological disorder, acquired, and inherited thrombophilias. The male partners of RPL couples were classified into two groups viz. RPL men (n = 8) with abnormal semen parameters (AS), RPL men (n = 51) with normal semen parameters (NS), and the third group was assigned to fertile controls (n = 20) with normal semen parameters (FC).

Y chromosome microdeletion analysis

In the Y chromosome microdeletion analysis, 54 blood samples were collected out of 59 males of couples with RPL (5 patients did not turn up for sampling) and they were screened for the presence of microdeletions using Y chromosome-specific sequence tagged site (STS) primer sets mentioned in [Table 1]. 14 blood samples from the collected 54 were rejected and could not be dissolved because of the delayed transportation and clotting. 11 STS loci spanning the long arm of Y chromosome (Yq) as shown in [Figure 1] were analyzed. STS sY14 was used to test for the presence of the short arm of the Y chromosome. Initial polymerase chain reaction (PCR) amplification was done in 20 µl of master mix containing 200 ng of genomic DNA, 2 ml of 1X PCR buffer, 20 µM dNTPs, varying concentrations of forward and reverse primers, and 2.5 unit of Brit Taq DNA-polymerase (Applied Biosystems, USA) [Table 2]. Initial denaturation at 95°C for 10 min was followed by 35 cycles of denaturation at 95°C for 30 s, annealing at 57°C for 45 s, and extension at 72°C for 45 s. A final extension was performed at 72°C for 10 min. The presence/absence of all the PCR fragments was tested in 2% agarose gels.
Table 1: PCR primer sequences for Y chromosome microdeletion

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Figure 1: Diagrammatic representation of the Y chromosome, the three azoospermia factor regions, and the location of the sequence tagged site loci used for analyzing Y chromosome microdeletions

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Table 2: Components of master mix (for 20 µ reaction volume)

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Semen analysis

Semen samples from the male partners were collected through masturbation after 3–4 days of sexual abstinence according to the WHO protocol.[16] The semen samples were allowed to liquefy at 37°C for 30 min. Physical examination such as liquefaction time, color, odor, and pH were recorded after 30 min. The basic microscopic examination was carried out to record the count, density, and motility of the sperm according to WHO protocol. DNA fragmentation index (DFI) and the hypo-osmotic swelling test could not be done due to nonavailability. Count, morphology, and motility were seen in RPL male patients and compared with fertile males.

Chi-square test was done to calculate the statistical significance where P < 0.05 were considered statistically significant.


   Results Top


59 cases were enrolled in study and after doing the recommended tests in females, (Cytogenetic, immunological, endocrinological, anatomical, and infectious); 62.7% (37/59) of cases remained inconclusive [Figure 2].
Figure 2: Distribution of RPM cases according to etiology

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Semen analysis was abnormal in 8 out of 59 cases (13.5%) and was exclusive in 5 (8.4%) cases, that is, 5 out of the 59 cases were exclusively attributable to abnormal semen analysis without Y chromosome microdeletion and without any co-existing factor. All 8 cases had decreased motility with 4 of them showing decreased count. Only 1 of them had antisperm antibodies. None had abnormal sperm morphology as per WHO criteria 2010 (in the sperm morphology abnormal head, tail, the middle piece, etc., were seen). DFI and the osmotic swelling test could not be done due to unequipped laboratories. Mean of sperm count in abnormal semen analysis (n = 8) and normal semen analysis (n = 51) in couples with RPL was 14.88 ± 11.23 and 38.38 ± 27.5, respectively whereas in FC (n = 20) was 42.20 ± 32.54 million/ml. Although, the sperm count was low in RPL cases, but the P value was insignificant compared to the FC. Motility was abnormal with a mean of 30.5% in all 8 RPM cases with abnormal semen analysis. Mean of motility with normal semen analysis (n = 51) in RPM was 41.88% ± 16.22% and in FC the mean of motility was 48.8% ± 18.15%. The motility was low in RPL cases compared to the fertile group but was statistically insignificant.

In our study, Y chromosome microdeletion was present in 13 out of 40 (32.5%) cases. Among these, it was exclusively positive in 8 patients (13.5%) after excluding recommended tests for a female. AZFa deletion was seen in 4 patients, AZFb in 1 patient, AZFc in 8 patients. [Figure 3]a and [Figure 3]b represent the multiplex PCR performed in patients and controls. The P value was significant, that is, 0.016 with microdeletion of Y chromosome in RPM cases as compared to 20 FC where no Y chromosome microdeletion was present [Table 3]. Only 3 cases had abnormal semen analysis co-existing with Y chromosome microdeletion. Cases with Y chromosome microdeleltion were categorized in [Table 4], showing the AZF location, corresponding normal or abnormal semen parameters and co-existing factors, if present. AZFa microdeletion was found in 4 out of 13, AZFb in 1, and AZFc in 8 out of the 13 positive cases.
Figure 3: (a) The figure represents the result of multiplex polymerase chain reactions tested in 2% agarose gels. M represents molecular weight marker lane while P and C represents patient and control lanes respectively (b) the figure represents the result of multiplex polymerase chain reactions on other sequence tagged site loci of patients (P) and controls (C)

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Table 3: Distribution of RPL cases according to semen analysis and Yq deletion compared to fertile controls

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Table 4: Distribution of Y chromosome microdeletion positive cases with respect to three AZF regions, semen analysis, and co-existing factors

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   Discussion Top


Recurrent miscarriage is a distressing event for the couple who desire for pregnancy. Clinical investigation and treatment are usually done after three miscarriages but can also be considered in couples with two consecutive miscarriages if:

  1. Embryonic heart activity observed in earlier pregnancy loss
  2. Normal karyotype on products of conception
  3. Female partner age over 35 years
  4. Infertility.
In 40% cases, there is no identifiable cause and are attributed to unknown endometrial disorders leading to placental insufficiency.[17] Approximately 70% of human conceptions fail to achieve viability with occult/preclinical/chemical loss as 50% (19% before implantation and 31% after implantation) and before 20 weeks (15%), respectively.

In our study, 59 couples with 3 or more miscarriage were recruited in the time span from August 2011 to June 2013. Couples were tested with recommended investigations of RPL. Additionally, Y -chromosome microdeletion and semen analysis was performed with a male partner as it contributes 50% to the conceptus but has been analyzed as a cause of RPL in limited studies. Three AZF regions on the long arm of Y chromosome are essential for normal spermatogenesis. AZF deletions have a negative impact on the sperm quality and abnormal spermatozoa that may be associated with RPL. The paternal genome provides the centrosome in thefirst mitotic division after fertilization. Sperm quality has been associated with the embryo's ability to reach the blastocyst stage and progress to implantation. Paternally expressed genes modulate the proliferation and invasiveness of trophoblast cells and later placental proliferation. Considering previous studies, the complete deletion of the AZFb and AZFc located on the Y chromosome may have a direct effect on early prophase of mitosis and decrease the rate of normal pairing in pachytene stage of spermatocytes.[6] Thus, this pairing failure may increase chromosomal abnormalities and could be related to recurrent pregnancy miscarriages.[17],[18] A study conducted on US population in 2006 found the frequency of 82% (14/17) in RPM cases, 20% (2/10) in infertile cases and none among 18 fertile cases.[6] The frequency of Y chromosome microdeletion in our study was 32.5% (13/40) and thus supports the findings of previous studies.

It is possible that the involvement of microdeletions in the etiology of RPL differs between populations. Different populations have different Y chromosome haplogroups, and it has been shown that the susceptibility to Y-chromosomal microdeletions varies between different haplogroups.[19] Studies done by Wettasinghe et al. in 2010 in Srilanka, Ghorbian et al. on Iranian population in 2012 and Venkatesh et al. in 2011 at AIIMS, New Delhi did not detect any microdeletion on Y chromosome in RPL cases suggesting variation between different haplogroups.[20],[21],[22] Our study is also comparable to study by Karaer et al. in Turkey [23] where 43 men from couples with recurrent pregnancy miscarriage and 43 men from couples with a live birth and no history of miscarriages were recruited and DNA was tested for the presence of 4 STSs spanning 4 AZF regions: DYS220 (AZFb), DYS235, DYS236, and DYS237. 16% (7/43) couples with recurrent pregnancy miscarriage had microdeletions in 1 or more of the 4 segments studied, whereas none of the fertile men had any microdeletions (P < 0.05). Their microdeletions were all found specifically at locus DYS 220 (AZFb). Despite the controversy over whether or not sperm quality is related to recurrent miscarriages, there are recent data which suggest that further studies into more specific sperm quality markers may be more revealing. The evidence is accumulating for paternal genome effect in early embryonic development as sperm integrity is vital for sperm–egg interactions, fertilization, and early embryonic development. Semen analysis in our study revealed abnormality in 13.6% (8/59) RPL cases as compared to the 20 fertile males (with no history of abortion) where none revealed any abnormality as per WHO criteria 2010. Although, the mean of count and motility was low in RPL cases, but P value was insignificant as compared to controls which supports the previous study.[24] There were no significant changes in sperm morphology in the present study as per WHO criteria for semen analysis 2010 which supports the previous study by Sbracia et al.[25] In our study, microdeletion of Y chromosome was present in 13 cases and co-existed with abnormal semen analysis in only 3 cases that is 7.5% (3/40) whereas semen analysis was normal in 10 of the cases with Y chromosome microdeletion.

Since etiology of RPL is multifactorial, both genetics, as well as environmental factors, contribute to its etiopathogenesis. In the present study, a significant association was found between Y -chromosome microdeletion in RPL cases and fertile couples. So, we recommend Y -chromosome microdeletion in patients with unexplained cause of RPL. The present data are preliminary, and further work is required to consider it as a routine part of the investigation in RPL and we may learn in near future how to treat some of these disorders. Currently, preimplantation genetic diagnosis is a method to evaluate embryos for aneuploidy. In the future, we may find other treatments that are more effective in treating couples with recurrent pregnancy miscarriage due to male factor.

What we investigate is only tip of the iceberg regarding many things which remain unexplained starting from ovum, sperm, zygote, implantation and acceptance of alloimmune environment affecting implantation which remains an unresolved mystery but we should always try to find out the etiology of correction and management. There are many etiological factors, and so there is no single therapy which may be applicable to all cases. Tender love and care are perceived as an important part in management specially where etiology remains inconclusive.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.

 
   References Top

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Hopps CV, Mielnik A, Goldstein M, Palermo GD, Rosenwaks Z, Schlegel PN. Detection of sperm in men with Y chromosome microdeletions of the AZFa, AZFb and AZFc regions. Hum Reprod 2003;18:1660-5.  Back to cited text no. 8
    
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Ferlin A, Arredi B, Speltra E, Cazzadore C, Selice R, Garolla A, et al.Molecular and clinical characterization of Y chromosome microdeletions in infertile men: A 10-year experience in Italy. J Clin Endocrinol Metab 2007;92:762-70.  Back to cited text no. 9
    
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Zini A, Meriano J, Kader K, Jarvi K, Laskin CA, Cadesky K. Potential adverse effect of sperm DNA damage on embryo quality after ICSI. Hum Reprod 2005;20:3476-80.  Back to cited text no. 12
    
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Moomjy M, Colombero LT, Veeck LL, Rosenwaks Z, Palermo GD. Sperm integrity is critical for normal mitotic division and early embryonic development. Mol Hum Reprod 1999;5:836-44.  Back to cited text no. 13
    
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Carrell DT, Hammoud SS. The human sperm epigenome and its potential role in embryonic development. Mol Hum Reprod 2010;16:37-47.  Back to cited text no. 14
    
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Evenson D, Wixon R. Meta-analysis of sperm DNA fragmentation using the sperm chromatin structure assay. Reprod Biomed Online 2006;12:466-72.  Back to cited text no. 15
    
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17.
Perrin J, Metzler-Guillemain C, Karsenty G, Grillo JM, Mitchell MJ, Guichaoua MR. Meiotic arrest at the midpachytene stage in a patient with complete azoospermia factor b deletion of the Y chromosome. Fertil Steril 2006;85:494.e5-8.  Back to cited text no. 17
    
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Geoffroy-Siraudin C, Aknin-Seiffer I, Metzler-Guillemain C, Ghalamoun-Slaimi R, Bonzi MF, Levy R, et al.Meiotic abnormalities in patients bearing complete AZFc deletion of Y chromosome. Hum Reprod 2007;22:1567-72.  Back to cited text no. 18
    
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Arredi B, Ferlin A, Speltra E, Bedin C, Zuccarello D, Ganz F, et al.Y-chromosome haplogroups and susceptibility to azoospermia factor c microdeletion in an Italian population. J Med Genet 2007;44:205-8.  Back to cited text no. 19
    
20.
Wettasinghe TK, Jayasekara RW, Dissanayake VH. Y chromosome microdeletions are not associated with spontaneous recurrent pregnancy loss in a Sinhalese population in Sri Lanka. Hum Reprod 2010;25:3152-6.  Back to cited text no. 20
    
21.
Ghorbian S, Saliminejad K, Sadeghi MR, Javadi GR, Kamali K, Amirjannati N, et al.The Association between Y chromosome microdeletion and recurrent pregnancy loss. Iran Red Crescent Med J 2012;14:358-62.  Back to cited text no. 21
    
22.
Venkatesh S, Thilagavathi J, Kumar K, Deka D, Talwar P, Dada R. Cytogenetic, Y chromosome microdeletion, sperm chromatin and oxidative stress analysis in male partners of couples experiencing recurrent spontaneous abortions. Arch Gynecol Obstet 2011;284:1577-84.  Back to cited text no. 22
    
23.
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[PUBMED]    
25.
Sbracia S, Cozza G, Grasso JA, Mastrone M, Scarpellini F. Semen parameters and sperm morphology in men in unexplained recurrent spontaneous abortion, before and during a 3 year follow-up period. Hum Reprod 1996;11:117-20.  Back to cited text no. 25
    


    Figures

  [Figure 1], [Figure 2], [Figure 3]
 
 
    Tables

  [Table 1], [Table 2], [Table 3], [Table 4]



 

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