Journal of Human Reproductive Science
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ORIGINAL ARTICLE Table of Contents   
Year : 2015  |  Volume : 8  |  Issue : 2  |  Page : 103-110
Adipose tissue-derived mesenchymal stem cells repair germinal cells of seminiferous tubules of busulfan-induced azoospermic rats


1 Stem Cell and Transgenic Technology Research Center, Shiraz, Iran
2 Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
3 Stem Cell and Transgenic Technology Research Center; Department of Medical Genetics, School of Medicine, Shiraz, Iran
4 Department of Hematology and Bone Marrow Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
5 Department of Biology, Islamic Azad University, Arsanjan Branch, Arsanjan, Iran
6 Laboratory Animal Center, Shiraz University of Medical Sciences, Shiraz, Iran

Correspondence Address:
Amin Tamadon
Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz
Iran
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0974-1208.158618

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Context: Adipose tissue-derived mesenchymal stem cells (AT-MSCs) are less invasive than bone marrow mesenchymal stem cells to obtain for cell therapy. Aims: The aims of this study were to evaluate the germinal cells characteristics and repairs in seminiferous tubules of busulfan-induced azoospermic rats after AT-MSCs transplantation. Settings and Design: Experimental case-control study. Materials and Methods: In the present experimental study, donors AT-MSCs were isolated from subcutaneous adipose tissue of two Sprague-Dawley rats. The recipients (n = 5) were received two doses of 10 mg/kg of busulfan with 21 days interval to stop endogenous spermatogenesis. After induction of azoospermia by busulfan, rats were injected with the AT-MSCs into the efferent duct of right testes. After 60 days, the right testes were injected AT-MSCs were compared to left azoospermic testes. Five untreated male rats served as negative control. Statistical Analysis Used: Stereological indices were analyzed by one-way ANOVA and LSD post-hoc test. The spermatogenesis index was compared using Mann-Whitney U test. Results: After stereological analyses, the seminiferous tubules treated with AT-MSCs had normal morphology. The untreated seminiferous tubules were empty. Spermatogenesis was observed in most cell-treated seminiferous tubules. Conclusions: The testis of busulfan-induced azoospermic rats accepted transplanted AT-MSCs. The transplanted AT-MSCs could induce spermatogenesis in seminiferous tubules of the rat.


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