Journal of Human Reproductive Science
Home Ahead of Print Current Issue Archives
   Bookmark this page Print this page Email this page Small font sizeDefault font size Increase font size    Users online: 334


 
ORIGINAL ARTICLE Table of Contents   
Year : 2013  |  Volume : 6  |  Issue : 1  |  Page : 54-58
The effects of honey and vitamin E administration on apoptosis in testes of rat exposed to noise stress


1 Fertility, Infertility and Perinatology Research Center, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
2 Physiology Research Center, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
3 Department of Immunology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Correspondence Address:
Ghasem Saki
Physiology Research Center, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, P.O. Box 61355 189, Ahvaz
Iran
Login to access the Email id

Source of Support: Research grant (n=PRC-92 ) from the Ahvaz Jundishapur University of Medical Sciences,, Conflict of Interest: None


DOI: 10.4103/0974-1208.112383

Rights and Permissions

Aims: A variety of stress factors are known to inhibit male reproductive functions. So this study was conducted in order to investigate the effects of honey and vitamin E on the germinative and somatic cells of testes of rats exposed to noise stress. Materials and Methods: Mature male wistar rats (n0 = 24) were randomly grouped as follows: Group 1 (honey + noise stress), 2 (vitamin E + noise stress), 3 (noise stress,) and 4 as the control group. In groups 1, 2, and 3, rats were exposed to noise stress. In groups 1 and 2, rats also were given honey and vitamin E, respectively, orally for 50 days. After that, the germinative and somatic cells of testes parenchyma were isolated by digesting the whole testes by a standard method. Next, viability, apoptosis, and necrosis of the cells were evaluated by TUNEL kit and flow cytometry. Results: The rates of apoptosis and necrosis of the testicular cells were increased (P = 0.003 and P = 0.001, respectively), but viability of these cells decreased in testes of rats exposed to noise stress (P = 0.003). However, administration of honey and vitamin E were significantly helpful in keeping the cells of testis parenchyma alive, which suffers from noise pollution (P < 0.05 and P < 0.05, respectively). Conclusions: Noise stress has negative influences on the cells of testicular tissue by increasing apoptotic and necrotic cells. However, the associated enhancement in healthy cells suggests that honey and vitamin E have positive influences on the testis parenchyma.


[FULL TEXT] [PDF]*
Print this article  Email this article
    

  Similar in PUBMED
    Search Pubmed for
    Search in Google Scholar for
  Related articles
   Citation Manager
  Access Statistics
   Reader Comments
   Email Alert *
   Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed2576    
    Printed88    
    Emailed0    
    PDF Downloaded141    
    Comments [Add]    

Recommend this journal