Journal of Human Reproductive Science
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ORIGINAL ARTICLE Table of Contents   
Year : 2010  |  Volume : 3  |  Issue : 1  |  Page : 32-34
 

Effect of forced swimming stress on in-vivo fertilization capacity of rat and subsequent offspring quality


1 Department of Anatomical Sciences, Laboratory of Cell Culture, Faculty of Medicine, Ahwaz Joundishapour University; Department of Anatomical Sciences, Physiology Research Center, Ahwaz Joundishapour University; and Apadana Clinical Research Center, Private Hospital of Apadana, Ahwaz
2 Department of Anatomical Sciences, Physiology Research Center, Ahwaz Joundishapour University, Ahwaz

Date of Submission01-Oct-2009
Date of Decision06-Nov-2009
Date of Acceptance04-Jan-2010
Date of Web Publication10-May-2010

Correspondence Address:
Fakher Rahim
Research Deputy, Ahwaz Jondishapour University of Medical Sciences, Ahwaz

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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0974-1208.63120

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   Abstract 

Aims: This study aimed to determine the effect of 50 days of forced swimming stress on fertilization capacity of rat and subsequent offspring quality. Setting and Design: The prospective study designed in vivo. Materials and Methods: Total 90 Wistar rats including 30 adult male (3 months of age, weighing 210 ± 10.6 g) and 60 female rats (3 months of age, weighing 230 ± 12.2 g) were engaged in this study. Male rats were randomly divided in two equal groups (n=15): Control and experimental groups. Animals of the experimental group were submitted to forced swimming stress for 3 min in water at 32oC daily for 50 days. Then all adult male rats were mated with normal females (2 per each male) for 7 days. Female rats were sacrificed and autopsy was performed on day 20 of pregnancy when uterus and ovaries were examined for the number of corpora lutea, dead and live fetuses, embryo resorption, implantation sites, and fetus weight. Conclusion: Results of this study have important implications for families attempting pregnancy. Stress pursuant to life events may have a negative impact on in vivo fertilization capacity of male rats and subsequent offspring quality.


Keywords: Fertilization capacity, in vivo fertilization, rat, stress


How to cite this article:
Saki G, Rahim F, Vaysi OA. Effect of forced swimming stress on in-vivo fertilization capacity of rat and subsequent offspring quality. J Hum Reprod Sci 2010;3:32-4

How to cite this URL:
Saki G, Rahim F, Vaysi OA. Effect of forced swimming stress on in-vivo fertilization capacity of rat and subsequent offspring quality. J Hum Reprod Sci [serial online] 2010 [cited 2019 Jul 23];3:32-4. Available from: http://www.jhrsonline.org/text.asp?2010/3/1/32/63120



   Introduction Top


Infertility is the inability of a couple to become pregnant after unprotected sexual intercourse (after 1 year). In the United States, about 10% of couples are affected by infertility. [1] According to the American Society for Reproductive Medicine, around 33% of the diagnosis is due to female infertility, 33% of the time it is linked to male infertility, and the rest 33% is due to a combination of factors from both partners. [1] The cause of the couple infertility cannot be determined in roughly 20% of the cases. [1] Psychological stress has been compromised as one of the major causes of idiopathic infertility in both men and women. [2],[3],[4],[5],[6],[7]

Many studies have investigated that psychological causes affect male factor infertility, [8],[9],[10],[11],[12],[13],[14] but it remains difficult to tease out stress as a cause or consequence of infertility. A diversity of stress factors such as micro-organisms, hyperthermia, and exposure to heavy metals inhibit male reproductive functions and spermatogenesis. [15] After implementation of the stressful stimuli such as prolonged immobilization and forced swimming stress, similar effects were observed. [16],[17] The previous study demonstrated that forced swimming stress in the time course equal spermatogenesis period, i.e., 48-50 days, [17] in the rat will be significantly effective to reduce the number and motility of sperm as well as the in vitro fertilization capacity. [18] The purpose of the present study was to determine whether forced swimming stress applied to adult male rat may affect in vivo fertilization capacity and subsequent offspring quality.


   Materials and Methods Top


This study was conducted from March 2009 to August 2009. A total 30 adult male Wistar rats, 3 months of age, weighing 210 ± 10.6 g were purchased from Laboratory Animals Care and Breeding Centre of Ahwaz Jondishapour University of Medical Sciences, Ahwaz, Iran. The fertilizing ability of male mice was proven at the beginning of the experiment by selecting post-first-wave of spermatogenesis that mate and observed positive pregnancy. [19] All rats were randomly divided into two equal groups (n = 15): Control and experimental groups. All animals were housed individually per cage under a 12-h light/dark cycle, 20 ± 2°C temperature and 60-65% humidity-controlled room with food and water ad libitum. All procedures were approved by international guidelines and by the Institute Research Ethics and Animal Care and Use Committee of Ahwaz Jondishapour University of Medical Sciences.

Experimental design

As the control group remained in their cages, the experimental group was submitted to forced swimming for 3 min in water at 32°C daily for 50 days. Stress was sized up by the hot-plate test after the last stressing session. [17] In the hot-plate, the plate temperature was 52°C and maximal cut-off time was 60 seconds. The latency time for the hind licking after exposure to the hot-plate surface was measured and the increase in relation to control was considered to be an index of the anti-nociceptive effect.

Fertility assessment

Adult control and stressed males were mated with sexually mature normal females presenting at least three regular cycles confirmed by the analysis of daily vaginal smears. Females in the pro-oestrus stage in the morning were mated with male rats overnight (two females per one male). The presence of spermatozoa in the vaginal smear on the next morning was indicative of copulation and was considered as day-zero of pregnancy. Autopsy was performed on day 20 of pregnancy when uterus and ovaries were examined for the number of corpora lutea, dead and live fetuses, and embryo resorption and implantation sites (total amount of live and dead fetuses plus embryo resorption). The rates of preimplantation loss (corpora lutea minus implantation sites) and postimplantation loss (implantation sites minus live fetuses) were then determined. [20]

Statistical analysis

Data are stated as a mean ± standard deviation (SD) and percentage. The statistical significance of difference between the control and experimental groups was determined by using the Chi-square. Differences between the mean were considered to be significant when P < 0.05 was achieved.


   Results Top


In the hot plate, 3 min swims at 328C significantly prolonged ( P < 0.05) the latency time for the hind paws latency (15.3 ± 1.66 s and 28.7 ± 2.33 s) for control and stressed rats [Table 1] . The pregnancy rate in female that mated with control and stressed male was 83% (25/30) and 60% (18/30 females), respectively. Statistical analysis showed significant differences between two groups ( P < 0.05). The numbers of live fetuses (as estimate male fertility rate) in female group that mated with control and stressed males were 6.4 ± 0.5 and 5 ± 0.2 per uterus, respectively. The numbers of live fetuses per uterus showed significant difference between two groups ( P < 0.05). There was no difference ( P > 0.05) in weight of live fetuses between two groups of study [Table 1]. Implantation sites were observed in 170 and 125 points of uteruses of pregnant female that mated with nonstress and stressed male rats, respectively. These values showed significant difference between two female groups ( P < 0.05). The rate of pre- and postimplantation loss in female mated with stressed male rats increased significantly (P < 0.05).


   Discussion Top


It is clear from the above observation that the forced swimming stress in rat will be effective to reduce in vivo fertilization capacity. The reduced fertilization capacity of stressed male is probably due to decrease in semen quality such as count, motility, and morphology. The association between psychosocial stress and decreased semen quality has biologic plausibility supported by numerous studies. Most hypothesized pathways by which stress may impact semen quality operate under endocrine factors. In animals, induced psychological stress negatively affects testosterone, LH levels, sexual behavior, and semen parameters. [21] It was previously reported that immobilization stress reduced the size and weight of testis as well as marked suppression of spermatogenesis and also suppressed all stages of cell division and maturity of spermatogenesis. [22] Moreover, in this study we observed that the rate of pre- and postimplantation loss in female rats mated with stressed male rats significantly increased. Almeida and coworker demonstrated that adult animals submitted to intermittent immobilization from prepuberty presented a significant decrease in fertility rates, confirmed by a more than twofold increase in both pre- and postimplantation loss in the progeny of normal females. [16] The cause of this manifestation is probably due to fertilization of oocytes with damaged spermatozoa. There are some reports about the deleterious effect of stress on animal and human semen quality such as a reduction in seminal volume, sperm concentration and motility, and in the amount of normal spermatozoa. [23],[24],[25] These manifestations are less obvious in men, being generally associated with extreme physical or psychological situation and are considered to be the basis of temporary infertility. [22]


   Conclusion Top


Present study demonstrates that forced swimming after the period of time necessary to complete an entire cycle of the spermatogenesis, between 48 and 53 days, when applied to adult male rats, the number of sperm, fertilization capacity, as well as motility of sperm will decrease significantly.

 
   References Top

1.Philippov OS, Radionchenko AA, Bolotova VP, Voronovskaya NI, Potemkina TV. Estimation of the prevalence and causes of infertility in western sibria. Bull Word Health Organ1998;76:183-7.  Back to cited text no. 1      
2.Shahid S. Depression in infertile couples. J Coll Physicians Surg Pak 2009;19:395-6.  Back to cited text no. 2  [PUBMED]    
3.Wischmann T, Scherg H, Strowitzki T, Verres R. Psychosocial characteristics of women and men attending infertility counseling. Hum Reprod 2009;24:378-85.   Back to cited text no. 3  [PUBMED]    
4.Akizuki Y, Kai I. Infertile Japanese women's perception of positive and negative social interactions within their social networks. Hum Reprod 2008;23:2737-43.  Back to cited text no. 4  [PUBMED]  [FULLTEXT]  
5.Damti OB, Sarid O, Sheiner E, Zilberstein T, Cwikel J. Stress and distress in infertility among women. Harefuah 2008;147:256-60, 276.  Back to cited text no. 5  [PUBMED]    
6.Moreira Sda N, Tertulino Fde F, Nogueira e Silva CA, Oliveira AG, Tomaz G, de Azevedo GD. Psicological aspects in infertile women. Acta Cir Bras 2005;20:152-8.  Back to cited text no. 6      
7.Sheiner EK, Sheiner E, Carel R, Potashnik G, Shoham-Vardi I. Potential association between male infertility and occupational psychological stress. J Occup Environ Med 2002;44:1093-9.  Back to cited text no. 7  [PUBMED]  [FULLTEXT]  
8.Zorn B, Auger J, Velikonja V, Kolbezen M, Meden-Vrtovec H. Psychological factors in male partners of infertile couples: Relationship with semen quality and early miscarriage. Int J Androl 2008;31:557-64.  Back to cited text no. 8  [PUBMED]  [FULLTEXT]  
9.Clarke RN, Klock SC, Geoghegan A, Travassos DE. Relationship between psychological stress and semen quality among in-vitro fertilization patients. Hum Reprod 1999;14:753-8.  Back to cited text no. 9  [PUBMED]  [FULLTEXT]  
10.Hjollund NH, Bonde JP, Henriksen TB, Giwercman A, Olsen J. Job strain and male fertility. Epidemiology 2004;15:114-7.  Back to cited text no. 10      
11.Sheiner E, Sheiner EK, Potashnik G, Carel R, Shoham-Vardi I. The relationship between occupational psychological stress and female fertility. Occup Med (Lond) 2003;53:265-9.  Back to cited text no. 11  [PUBMED]  [FULLTEXT]  
12.Hjollund NH, Bonde JP, Henriksen TB, Giwercman A, Olsen J. Reproductive effects of male psychologic stress. Epidemiology 2004;15:21-7.  Back to cited text no. 12      
13.Pook M, Krause W, Rohrle B. Coping with infertility: Distress and changes in sperm quality. Hum Reprod 1999;14:1487-92.  Back to cited text no. 13      
14.Pook M, Tuschen-Caffier B, Kubek J, Schill WB, Krause W. Personality, coping and sperm count. Andrologia 2005;37:29-35.  Back to cited text no. 14  [PUBMED]  [FULLTEXT]  
15.Ozawa N, Goda N, Makino N, Yamaguchi T, Yoshimura Y, Suematsu M. Leydig cell-derived heme oxygenase-1 regulates apoptosis of premeiotic germ cells in response to stress. J Clin Invest 2002;109:457-67.  Back to cited text no. 15      
16.Almeida AS, Kempinas WG, Lamano Carvalho TL. Sexual behavior and fertility of male rats submitted to prolonged immobilization-induced stress. Braz J Med Biol Res 2000;33:1105-9.  Back to cited text no. 16      
17.Mingoti GZ, Pereita RN, Monteiro CM. Fertility of male adult rats submitted to forced swimming stress. Braz J Med Biol Res 2003;36:677- 81.  Back to cited text no. 17      
18.Potemina TE. Impairment of spermatogenesis in male rats during stress. Bull Exp Biol Med 2008;145:700-2.  Back to cited text no. 18  [PUBMED]    
19.Movassaghi S, Saki G, Javadnia F, Panahi P, Mahmoudi M, Rhim F. Effects of methyl-beta-cyclodextrin and cholesterol on cryosurvival of spermatozoa from C57BL/6 mouse. Pak J Biol Sci 2009;12:19-25.  Back to cited text no. 19      
20.Saki G, Safikhani Z, Sobhani A, Salehnia M. The in vitro fertilization rate of mouse ova in the absence or presence of recombinant human leukemia inhibitory factor. Pak J Med Sci 2006;22:438-41.  Back to cited text no. 20      
21.Almeida SA, Anselmo-Franci JA, Rosa e Silva AA, Carvalho TL. Chronic intermittent immobilization of male rats throughout sexual development: A stress protocol. Exp Physiol 1998;83:701-4.  Back to cited text no. 21  [PUBMED]  [FULLTEXT]  
22.Rai, J, Pandey SN, Srivastava RK. Effect of immobilization stress on spermatogenesis of albino rats. J Anat Soc India 2003;52:52-7.  Back to cited text no. 22      
23.Steinberger E. The etiology and pathophysiology of testicular dysfunction in man. Fertil Steril 1978;29:481-91.  Back to cited text no. 23  [PUBMED]    
24.Giblin PT, Poland ML, Moghissi KS, Ager JW, Olson JM. Effect of stress and characteristic adaptability on semen quality in healthy men. Fertil Steril 1988;49:127-32.  Back to cited text no. 24  [PUBMED]    
25.Almeida AS, Petenusci SO, Anseimo-Franci JA, Rosa-e-Silva AA, Lamano Carvalho TL. Decreased spermatogenic and androgenic testicular functions in adult rats submitted to immobilization-induced stress from prepuberty. Braz J Med Biol Res 1998;31:1443-8.  Back to cited text no. 25      



 
 
    Tables

  [Table 1]


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