Journal of Human Reproductive Science
Home Ahead of Print Current Issue Archives
   Bookmark this page Print this page Email this page Small font sizeDefault font size Increase font size    Users online: 37


 
ORIGINAL ARTICLE Table of Contents   
Year : 2009  |  Volume : 2  |  Issue : 2  |  Page : 72-75
Effect of forced swimming stress on count, motility and fertilization capacity of the sperm in adult rats


1 Department of Laboratory of Cell Culture, Anatomical Sciences, Faculty of Medicine; Physiology Research Center, Joundishapour University; Apadana Clinical Research Center, Private Hospital of Apadana, Ahwaz, Iran
2 Apadana Clinical Research Center, Private Hospital of Apadana, Ahwaz, Iran

Correspondence Address:
Fakher Rahim
Research Deputy, Ahwaz Jondishapour University of Medical Sciences, Ahwaz
Iran
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0974-1208.57226

Rights and Permissions

Aims: The purpose of this study was to determine whether 50 days of forced swimming stress applied to adult male rats affects count, motility and fertilization capacity of sperm. Settings and Design: It is a prospective study designed in vitro. Materials and Methods: A total 30 adult male wistar rats were used in this study. All rats were divided into two equal groups (n = 15): (1) control group and (2) experimental group. Animals of the experimental group were submitted to force swimming stress for 3 min in water at 32°C daily for 50 days. Then, all male rats were sacrificed, the right epididymides were removed and sperm concentration and motility were determined. The sperm suspension was added to the ova. Fertilization capacity was assessed by counting two-cell embryos 24-26 h after completion of fertilization in vitro. Statistical Analysis Used: Data are reported as mean ± SD and percentage. The difference between the control and experimental groups was determined by the unpaired t-test. Results: The mean and standard deviation of sperm concentration in the control and experimental groups were 60.8 ± 9.3 10 6 /ml and 20.4 ± 5.3 10 6 /ml, respectively. There was a statistical difference of P < 0.05 between the two groups in terms of sperm concentration. The percentage of motility in the experimental group was significantly different ( P < 0.05). The same results were obtained in case of fertility ( P < 0.05). Stress caused by forced swimming was observed by a significant increase in the latency of the pain response in the hot-plate test ( P < 0.05). Conclusions: These results suggest that forced swimming stress in time course equal or more than spermatogenesis period, i.e. 48-50 days in the rat will be significantly effective to reduce the number and motility of sperms as well as the fertilization capacity.


[FULL TEXT] [PDF]*
Print this article  Email this article
    

  Similar in PUBMED
    Search Pubmed for
    Search in Google Scholar for
  Related articles
   Citation Manager
  Access Statistics
   Reader Comments
   Email Alert *
   Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed5410    
    Printed203    
    Emailed0    
    PDF Downloaded682    
    Comments [Add]    
    Cited by others 17    

Recommend this journal